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Polyploid Germplasm Induction of Bletilla ochracea Schltr.
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Jiamin HE1, 2, Sujin ZHENG2, 3, Xinhua ZENG2, 3, Shaofan LUO2, Siren LAN1, Weichang HUANG1, 2, 3, *
Chinese Journal of Tropical Crops | 2025, 46(4) : 887 - 893
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Chinese Journal of Tropical Crops | 2025, 46(4): 887-893
• Germplasm Resources, Genetics & Breeding •
Polyploid Germplasm Induction of Bletilla ochracea Schltr.
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Jiamin HE1, 2, Sujin ZHENG2, 3, Xinhua ZENG2, 3, Shaofan LUO2, Siren LAN1, Weichang HUANG1, 2, 3, *
Affiliations
  • 1.College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
  • 2.Shanghai Chenshan Botanical Garden / Eastern China Conservation Centre for Wild Endangered Plant Resources, Shanghai 201602, China
  • 3.Shanghai Chenshan Botanical Garden / Shanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai 201602, China
Published: 2025-04-25 doi: 10.3969/j.issn.1000-2561.2025.04.011
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To generate polyploid B. ochracea Schltr and find out the optimal induction condition, the seeds of diploid B. ochracea were treated with different concentration of colchicine solution under a range of treatment time. The results of the study suggested that the seeds treated with 0.25% colchicine solution for six days was effective in polyploid induction for B. ochracea, with a 35.66% survival rate of treated seeds and a 45% induction rate. The tetraploid gave a significant enhancement on various agronomic traits, including plant height, leaf number, leaf width, stem diameter, bulb length and root diameter. In addition, tetraploid B. ochracea showed increased stomatal aperture and decreased stomatal density. The tetraploid B. ochracea was generated in this study, which would provide technical support and basic material for improving the genetic traits of medicinal orchids.

Bletilla ochracea Schltr.  /  polyploidy  /  colchicine  /  flow cytometer  /  chromosome counting
Jiamin HE, Sujin ZHENG, Xinhua ZENG, Shaofan LUO, Siren LAN, Weichang HUANG. Polyploid Germplasm Induction of Bletilla ochracea Schltr.[J]. Chinese Journal of Tropical Crops, 2025 , 46 (4) : 887 -893 . DOI: 10.3969/j.issn.1000-2561.2025.04.011
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Article Info
doi: 10.3969/j.issn.1000-2561.2025.04.011
  • Receive Date：2024-11-27
  • Online Date：2026-06-24
  • Published：2025-04-25
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  • Received：2024-11-27
  • Accepted：2024-12-05
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Affiliations
    1.College of Landscape Architecture, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China
    2.Shanghai Chenshan Botanical Garden / Eastern China Conservation Centre for Wild Endangered Plant Resources, Shanghai 201602, China
    3.Shanghai Chenshan Botanical Garden / Shanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai 201602, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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