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Cloning, Localization and Expression Analysis of MiERF20 Gene in Mango Fruit
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Pan GUO1, 2, Xin WANG1, 2, Shasha ZHANG1, 2, Dexin MA3, Yan GONG3, Yuanzhi SHAO1, 2, Wen LI1, 2, *
Chinese Journal of Tropical Crops | 2025, 46(4) : 788 - 797
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Chinese Journal of Tropical Crops | 2025, 46(4): 788-797
• Omics & Biotechnology •
Cloning, Localization and Expression Analysis of MiERF20 Gene in Mango Fruit
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Pan GUO1, 2, Xin WANG1, 2, Shasha ZHANG1, 2, Dexin MA3, Yan GONG3, Yuanzhi SHAO1, 2, Wen LI1, 2, *
Affiliations
  • 1.School of Tropical Agriculture and Forestry, Hainan University, Danzhou, Hainan 571737, China
  • 2.Sanya Institute of Southern Propagation, Hainan University, Sanya, Hainan 572025, China
  • 3.Hainan Haiken Cold Chain Development Co., Ltd., Sanya, Hainan 572013, China
Published: 2025-04-25 doi: 10.3969/j.issn.1000-2561.2025.04.002
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Mango (Mangifera indica L.), as a typical climacteric fruit, undergoes ripening and senescence processes closely related to ethylene. Ethylene-responsive factors (ERF) play crucial roles in plant growth, fruit ripening and ethylene signaling transduction. Mango variety Guifei was used, MiERF20, 492 bp long and encoding 163 amino acids, was cloned and analyzed. Bioinformatics analysis revealed that MiERF20 was an unstable, hydrophilic protein without signal peptides or transmembrane regions, containing 25 phosphorylation sites and one AP2 conserved domain. The secondary structure was dominated by 40.49% irregular coils, 33.13% α-helices, 16.56% extensions and 9.82% β-rotations. Its secondary structure comprised irregular coils, α-helices, extended chains, and β-turns, consistent with AP2 domain features. MiERF20 showed 83.89% homology with ERF20 of Lacertidae pistachio. Subcellular localization indicated that MiERF20 was located in the nucleus and possessed transcriptional activation activity. Prokaryotic expression assays demonstrated that the protein could be inducibly expressed in a prokaryotic system. Real-time fluorescence quantification showed that MiERF20 was ethylene-inducible, suggesting its role in ethylene-regulated fruit ripening. This study would provide a foundation for further analysis of the functions and regulatory mechanisms of ERF transcription factors during postharvest fruit ripening.

mango  /  ERF transcription factor  /  ethylene signaling  /  gene expression  /  expression analysis
Pan GUO, Xin WANG, Shasha ZHANG, Dexin MA, Yan GONG, Yuanzhi SHAO, Wen LI. Cloning, Localization and Expression Analysis of MiERF20 Gene in Mango Fruit[J]. Chinese Journal of Tropical Crops, 2025 , 46 (4) : 788 -797 . DOI: 10.3969/j.issn.1000-2561.2025.04.002
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Year 2025 volume 46 Issue 4
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Article Info
doi: 10.3969/j.issn.1000-2561.2025.04.002
  • Receive Date：2024-11-19
  • Online Date：2026-06-24
  • Published：2025-04-25
Article Data
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History
  • Received：2024-11-19
  • Accepted：2024-12-18
Funding
Affiliations
    1.School of Tropical Agriculture and Forestry, Hainan University, Danzhou, Hainan 571737, China
    2.Sanya Institute of Southern Propagation, Hainan University, Sanya, Hainan 572025, China
    3.Hainan Haiken Cold Chain Development Co., Ltd., Sanya, Hainan 572013, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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