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Seed shattering is the main reason for the continuous self-reproduction and spread of weedy rice in rice fields. Genes SH4 and qSH1 are considered to be the main genes controlling rice seed shattering. The aim of this study was to determine the genotype of the seed shattering SNP locus at SH4 and qSH1 of weedy rice in Hainan. Using weedy rice from rice fields in Lingao and Yazhou District as research materials, this study collected weedy rice and corresponding cultivated rice seeds from 13 rice fields. 144 Weedy rice seeds with different phenotypes and 13 corresponding cultivated rice seeds were selected for germination and planting, and the seed shattering rates were measured. PCR amplification and sequence analysis were performed on the seed shattering SNP locus at SH4 and qSH1. The results showed that there were 77 wild type (G) weedy rice plants, 48 mutant type (T) weedy rice plants, and 19 heterozygous type (G/T) weedy rice plants with the locus at SH4 gene; Seed shattering SNP locus of SH4 gene in all cultivated rice samples were T. The determination of seed shattering rate found that 96 weedy rice samples with G and G/T genotype seed shattering SNP at SH4 gene had extremely high seed shattering rates, while among 48 weedy rice samples with T genotype seed shattering SNP locus at SH4 gene, 40 weedy rice samples had moderate seed shattering rates and 8 weedy rice samples had low seed shattering rates. Seed shattering SNP locus of qSH1 in all weedy rice and cultivated rice samples were wild-type seed shattering G genotype. The above results indicate that the difference in seed shattering rate between Hainan weedy rice and cultivated rice is not related to the seed shattering SNP locus at qSH1 gene, but significantly correlated with the seed shattering SNP locus at SH4 gene. The difference in seed shattering rate between weedy rice and cultivated rice with T genotype SNP locus at SH4 gene may be caused by differences in other unknown genes or locus. This study would provide a molecular basis for the seed shattering mechanism of weedy rice in Hainan.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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