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GASA (gibberellic acid-stimulated Arabidopsis) is a small molecule polypeptide widely presented in plants, playing an important role in plant growth and development processes. It has potential value especially in the regulation of mulberry bud dormancy. However, currently, the understanding of the functions of MaGASA genes in mulberry during the bud dormancy process is limited. The study aimed to identify the members of the mulberry GASA gene family and analyze the roles in bud dormancy. Bioinformatics methods were used to identify the members and analyze the physicochemical properties and other characteristics. Meanwhile, real-time fluorescence quantitative PCR and transcriptome data were utilized to analyze the expression patterns. The results showed that there were 12 MaGASA family members (MaGASA1-MaGASA12) in mulberry, which were distributed on eight chromosomes. There were differences in acidity and alkalinity among the members, and the average hydrophilicity coefficients were positive or negative, but all contained the GASA conserved domain. The phylogenetic tree showed that they had a close genetic relationship with peach. Collinearity analysis revealed that there were three collinear relationships in the MaGASA gene family. The promoter regions of MaGASAs contained cis-acting elements that respond to light, low temperature, and various hormones. The MaGASA family members showed tissue-variety specific expression. Among them, the expression level of MaGASA12 in mulberry buds was significantly higher than that in other parts. Transcriptome analysis indicated that the expression level of MaGASA12 decreased significantly during the dormancy breaking process, while the expression level of MaGASA10 increased sharply during the dormancy breaking process. The expression levels of MaGASA10 and MaGASA12 at different dormancy stages were detected by real-time fluorescence quantitative PCR technology, and the results further verified the conclusions of the transcriptome analysis, indicating that they may be related to the winter bud dormancy process of Morus. The research results would provide a theoretical reference for the in-depth study of the biological functions of MaGASA in mulberry and genetic breeding.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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