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Screening and Validation of MeVINV1 Interacting Proteins in Cassava
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Dongqing FU1, 2, 3, Xiangwen WANG1, 2, 3, Yujian GE1, 2, 3, Chen SHEN2, 3, 4, Jinpeng CHEN1, 3, Yuan YAO2, 3, Mengting GENG1, Xiaohua LU2, 3, *, Yajie WANG2, 3, *
Chinese Journal of Tropical Crops | 2025, 46(6) : 1297 - 1306
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Chinese Journal of Tropical Crops | 2025, 46(6): 1297-1306
• Omics & Biotechnology •
Screening and Validation of MeVINV1 Interacting Proteins in Cassava
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Dongqing FU1, 2, 3, Xiangwen WANG1, 2, 3, Yujian GE1, 2, 3, Chen SHEN2, 3, 4, Jinpeng CHEN1, 3, Yuan YAO2, 3, Mengting GENG1, Xiaohua LU2, 3, *, Yajie WANG2, 3, *
Affiliations
  • 1.School of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China
  • 2.Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences / Hainan Institute for Tropical Agricultural, Haikou, Hainan 571101, China
  • 3.Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya, Hainan 572000, China
  • 4.College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China
Published: 2025-06-25 doi: 10.3969/j.issn.1000-2561.2025.06.003
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Cassava (Manihot esculenta Crantz), the “king of starch”, is an important tropical food crop and a staple food for one billion people worldwide. Cassava vacuolar invertase (MeVINV1) is involved in plant growth and development and stress defense by irreversibly breaking down sucrose into glucose and fructose. Eight candidate proteins interacting with MeVINV1 were screened from the cassava SC8 cDNA yeast library by yeast two-hybrid experiment to explore the gene function and molecular regulatory network of MeVINV1. Furthermore, yeast point-to-point verification found that MeVINV1 interacted with MeUN-3, MeMTP1, MeCASP4A3 and MePIRL3, and the interaction with MeMTP1 was stronger. Therefore, it is speculated that MeVINV1 and MeMTP1 synergistically regulate vacuolar sucrose metabolism and metal ion transport, and participate in the regulation of cassava response to metal ion stress. The results of the study would provide a scientific basis for providing new genetic resources and molecular mechanisms of stress regulation in cassava stress resistance breeding.

cassava  /  MeVINV1  /  yeast two-hybrid  /  interaction protein validation
Dongqing FU, Xiangwen WANG, Yujian GE, Chen SHEN, Jinpeng CHEN, Yuan YAO, Mengting GENG, Xiaohua LU, Yajie WANG. Screening and Validation of MeVINV1 Interacting Proteins in Cassava[J]. Chinese Journal of Tropical Crops, 2025 , 46 (6) : 1297 -1306 . DOI: 10.3969/j.issn.1000-2561.2025.06.003
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Article Info
doi: 10.3969/j.issn.1000-2561.2025.06.003
  • Receive Date：2025-01-20
  • Online Date：2026-06-24
  • Published：2025-06-25
Article Data
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History
  • Received：2025-01-20
  • Accepted：2025-02-12
Funding
Affiliations
    1.School of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China
    2.Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences / Hainan Institute for Tropical Agricultural, Haikou, Hainan 571101, China
    3.Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya, Hainan 572000, China
    4.College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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