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Screening of Interacting Proteins of Cassava Isoamylase MeISA2
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Yujian GE1, 2, 3, Dongqing FU1, 2, 3, Xiangwen WANG1, 2, 3, Chen SHEN2, 3, 4, Yuheng LI1, 2, Yuan YAO2, 3, Mengting GENG1, Xiaohua LU2, 3, *, Yajie WANG2, 3, *
Chinese Journal of Tropical Crops | 2025, 46(6) : 1322 - 1329
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Chinese Journal of Tropical Crops | 2025, 46(6): 1322-1329
• Omics & Biotechnology •
Screening of Interacting Proteins of Cassava Isoamylase MeISA2
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Yujian GE1, 2, 3, Dongqing FU1, 2, 3, Xiangwen WANG1, 2, 3, Chen SHEN2, 3, 4, Yuheng LI1, 2, Yuan YAO2, 3, Mengting GENG1, Xiaohua LU2, 3, *, Yajie WANG2, 3, *
Affiliations
  • 1.School of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China
  • 2.Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture and Rural Affairs / Hainan Institute for Tropical Agricultural Resources / Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province, Haikou, Hainan 571101, China
  • 3.Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya, Hainan 572000, China
  • 4.College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China
Published: 2025-06-25 doi: 10.3969/j.issn.1000-2561.2025.06.005
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Isoamylase (Isoamylase, ISA) catalyzes the hydrolysis of α-1,6 linkages, thereby affecting the branching structure of starch and promoting crystallization and granule formation. The study utilized yeast two-hybrid screening to identify proteins interacting with MeISA2 to explore new proteins that may interact with MeISA2 to influence cassava starch accumulation. A bait protein vector pGBKT7-MeISA2 was constructed, and it was demonstrated that MeISA2 did not possess autoactivation activity and was non-toxic to yeast cells. Through yeast two-hybrid screening of a cDNA library, a total of 7 candidate interacting proteins were obtained. Point-to-point yeast two-hybrid re-validation experiments confirmed the interaction between MeISA2 and MePsbO1. MePsbO1 belongs to the Photosystem II oxygen-evolving enhancer protein (PsbO) family, which facilitates the efficient operation of the photosynthetic electron transfer process. Bioinformatics analysis revealed that MePsbO1 had the closest phylogenetic relationship with HbPsbO1 from Hevea brasiliensis, a member of the Euphorbiaceae family, with a similarity of 90.12%, while its similarity to Arabidopsis thaliana AtPsbO1 was only 77.71%. It is predicted that MePsbO1 is localized in the chloroplast, and the MePsbO1 gene is predominantly expressed in leaves. Plant ISA is involved in the synthesis of transient leaf starch, thus it is hypothesized that MePsbO1 and MeISA2 may interact to jointly regulate the rate of photosynthesis and the accumulation of transient leaf starch. The results study would provide new insights into the mechanism by which the MeISA2 gene participates in the accumulation and distribution of photosynthetic products in cassava source organs.

cassava  /  MeISA2  /  yeast two-hybrid  /  MePsbO1
Yujian GE, Dongqing FU, Xiangwen WANG, Chen SHEN, Yuheng LI, Yuan YAO, Mengting GENG, Xiaohua LU, Yajie WANG. Screening of Interacting Proteins of Cassava Isoamylase MeISA2[J]. Chinese Journal of Tropical Crops, 2025 , 46 (6) : 1322 -1329 . DOI: 10.3969/j.issn.1000-2561.2025.06.005
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Article Info
doi: 10.3969/j.issn.1000-2561.2025.06.005
  • Receive Date：2025-01-21
  • Online Date：2026-06-24
  • Published：2025-06-25
Article Data
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History
  • Received：2025-01-21
  • Accepted：2025-02-17
Funding
Affiliations
    1.School of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan 570228, China
    2.Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture and Rural Affairs / Hainan Institute for Tropical Agricultural Resources / Key Laboratory for Biology and Genetic Resources of Tropical Crops of Hainan Province, Haikou, Hainan 571101, China
    3.Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya, Hainan 572000, China
    4.College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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