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Identification of Members of the LAV Subfamily in Woody Oil Crops Plukenetia volubilis L. and the Function of Regeneration-related Gene PvoLEC2
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Shiling DENG1, 2, Jinhui MO1, 2, Jingjing YU2, Yingda CHEN2, 3, Shitao MA1, 2, Yuandong LU1, 2, Yi WANG1, 2, *
Chinese Journal of Tropical Crops | 2025, 46(6) : 1330 - 1340
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Chinese Journal of Tropical Crops | 2025, 46(6): 1330-1340
• Omics & Biotechnology •
Identification of Members of the LAV Subfamily in Woody Oil Crops Plukenetia volubilis L. and the Function of Regeneration-related Gene PvoLEC2
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Shiling DENG1, 2, Jinhui MO1, 2, Jingjing YU2, Yingda CHEN2, 3, Shitao MA1, 2, Yuandong LU1, 2, Yi WANG1, 2, *
Affiliations
  • 1.Key Laboratory of Fast-growing Timber Breeding in Central and South China, National Forestry and Grassland Administration, Nanning, Guangxi 530000, China
  • 2.College of Forestry, Guangxi University, Nanning, Guangxi 530000, China
  • 3.Key Laboratory of Cultivation and Utilisation of Subtropical Plantation Forests in Guangxi Colleges and Universities, Nanning, Guangxi 530000, China
Published: 2025-06-25 doi: 10.3969/j.issn.1000-2561.2025.06.006
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LEC2 is an important member of the LAV subfamily in the plant B3 superfamily and plays a significant regulatory role in embryo development and meristem maintenance. To explore the function of the PvoLEC2 in the cell regeneration of Plukenetia volubilis, the genomic sequence of PvoLEC2 (gPvoLEC2) was cloned through phylogenetic analysis and molecular biology techniques. The function of gPvoLEC2 in promoting plant regeneration was verified in Nicotiana benthamiana, and the function of gPvoLEC2 was studied in P. volubilis hairy roots. Phylogenetic and gene structure analysis indicated that the LEC2-ABI3 subfamily of Euphorbiaceae plants such as P. volubilis, and Arabidopsis thaliana, Oryza sativa, Populus spp. were relatively conserved. Expression analysis showed that PvoLEC2 was only weakly expressed in the late stage of P. volubilis seed development (50-65 DAP), and the motifs and key amino acid sites of the B3 domain were conserved. Overexpression of gPvoLEC2 led to dwarfing of transgenic N. benthamiana plants, difficulty in the growth of the main stem, and abnormal leaf development. At the same time, the regeneration ability of gPvoLEC2 transgenic N. benthamiana organs in vitro was significantly higher than that of the wild type, but lower than that of the positive control-ZmWUS2 & STM transgenic N. benthamiana. Overexpression of gPvoLEC2 in P. volubilis hairy roots led to their shortening and thickening. The results indicate that the genomic sequence of PvoLEC2 is a functional sequence that promotes plant regeneration, but it also causes abnormal development.

Plukenetia volubilis L.  /  PvoLEC2  /  LAV subfamily  /  plant regeneration  /  Nicotiana benthamiana
Shiling DENG, Jinhui MO, Jingjing YU, Yingda CHEN, Shitao MA, Yuandong LU, Yi WANG. Identification of Members of the LAV Subfamily in Woody Oil Crops Plukenetia volubilis L. and the Function of Regeneration-related Gene PvoLEC2[J]. Chinese Journal of Tropical Crops, 2025 , 46 (6) : 1330 -1340 . DOI: 10.3969/j.issn.1000-2561.2025.06.006
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Article Info
doi: 10.3969/j.issn.1000-2561.2025.06.006
  • Receive Date：2025-01-07
  • Online Date：2026-06-24
  • Published：2025-06-25
Article Data
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  • Received：2025-01-07
  • Accepted：2025-02-12
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Affiliations
    1.Key Laboratory of Fast-growing Timber Breeding in Central and South China, National Forestry and Grassland Administration, Nanning, Guangxi 530000, China
    2.College of Forestry, Guangxi University, Nanning, Guangxi 530000, China
    3.Key Laboratory of Cultivation and Utilisation of Subtropical Plantation Forests in Guangxi Colleges and Universities, Nanning, Guangxi 530000, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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