Home Journals Articles Updates About
CN
image
收藏切换
Cloning and Expression Analysis of the Biological Clock Gene OnELF3 in Oncidium
收藏切换
PDF
Yurong TAN1, 2, 3, Xiaohua PEI1, 2, Ke ZHAI1, 2, Shuang WU1, 2, Mengtao LI1, 2, Qingchang MENG1, 2, Haoming SONG1, 2, Yujing YANG1, 2, Yingwen PAN4, Guangming XIE1, 2, 5, Xiumei LI5, Jinping LIU1, 2, *
Chinese Journal of Tropical Crops | 2025, 46(2) : 292 - 299
Less
收藏切换
Chinese Journal of Tropical Crops | 2025, 46(2): 292-299
• Omics & Biotechnology •
Cloning and Expression Analysis of the Biological Clock Gene OnELF3 in Oncidium
Full
Yurong TAN1, 2, 3, Xiaohua PEI1, 2, Ke ZHAI1, 2, Shuang WU1, 2, Mengtao LI1, 2, Qingchang MENG1, 2, Haoming SONG1, 2, Yujing YANG1, 2, Yingwen PAN4, Guangming XIE1, 2, 5, Xiumei LI5, Jinping LIU1, 2, *
Affiliations
  • 1.School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya, Hainan 572024, China
  • 2.School of Tropical Agriculture and Forestry, Hainan University, Danzhou, Hainan 571737, China
  • 3.Guangxi Academy of Argicultural Sciences, Nanning, Guangxi 530007, China
  • 4.Post-entry Quarantine Station for Tropical Plant, Hainan Entry-exit Inspection and Quarantine Bureau, Haikou, Hainan 570311, China
  • 5.Wuzhishan Wanquan Horticulture Co., Ltd. / Hongshan Farm, Zhongshan Town, Wuzhishan, Hainan 572216, China
Published: 2025-02-25 doi: 10.3969/j.issn.1000-2561.2025.02.005
Outline
收藏切换

ELF3 is an important biological clock gene that regulates the input of light signal and the output of circadian rhythm, participating in modulation of many important processes of plants. In order to understand its regulation in the initiation of petal senescence of Oncidium, we conducted cloning and expression analysis of OnELF3 in Oncidium.OnELF3 (GenBank accession number: OL613896) was isolated from the Oncidium petals. OnELF3 had a length of 2208 bp and encoded a protein of 735 amino acids. Subcellular localization showed its expression in the nucleus. Homologous sequence alignment and phylogenetic tree analysis showed that OnELF3 was closest to the counterparts of Dendrobium candidum and Phalaenopsis. Prokaryotic expression analysis showed that the cloned OnELF3 was capable of prokaryotic expression of functional proteins. Its expression patterns under long day, full light, and full darkness conditions were consistent with its function as a circadian clock gene. Real time fluorescence quantitative analysis showed that the expression of OnELF3 was the highest in cracked buds and full-open flowers, indicating that OnELF3 may play a role in both flowering and flower senescence initiation. This study would lay a foundation for further functional identification of OnELF3 in the initiation of flower senescence.

Oncidium  /  flower senescence  /  ELF3 gene  /  gene cloning  /  expression analysis
Yurong TAN, Xiaohua PEI, Ke ZHAI, Shuang WU, Mengtao LI, Qingchang MENG, Haoming SONG, Yujing YANG, Yingwen PAN, Guangming XIE, Xiumei LI, Jinping LIU. Cloning and Expression Analysis of the Biological Clock Gene OnELF3 in Oncidium[J]. Chinese Journal of Tropical Crops, 2025 , 46 (2) : 292 -299 . DOI: 10.3969/j.issn.1000-2561.2025.02.005
Appendix
Less
Year 2025 volume 46 Issue 2
PDF
64
32
Cite this Article
BibTeX
Article Info
doi: 10.3969/j.issn.1000-2561.2025.02.005
  • Receive Date：2024-09-05
  • Online Date：2026-06-23
  • Published：2025-02-25
Article Data
Affiliations
History
  • Received：2024-09-05
  • Revised：2024-09-28
Funding
Affiliations
    1.School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan University, Sanya, Hainan 572024, China
    2.School of Tropical Agriculture and Forestry, Hainan University, Danzhou, Hainan 571737, China
    3.Guangxi Academy of Argicultural Sciences, Nanning, Guangxi 530007, China
    4.Post-entry Quarantine Station for Tropical Plant, Hainan Entry-exit Inspection and Quarantine Bureau, Haikou, Hainan 570311, China
    5.Wuzhishan Wanquan Horticulture Co., Ltd. / Hongshan Farm, Zhongshan Town, Wuzhishan, Hainan 572216, China
References
Share
https://castjournals.cast.org.cn/joweb/rdzwxb/EN/10.3969/j.issn.1000-2561.2025.02.005
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House