In this study, the rhizosphere soil of jasmine was used as the material under the condition of artificial greenhouse to analyze and compare the effects of two fungicides (fluazinam and tebuconazole) on soil physical and chemical properties, soil enzyme activities in addition to carbon source metabolic diversity of soil microbial community. The indexes of soil physical and chemical properties were different under the application of the two fungicides of different concentration. The pH value and organic matter content of soil decreased by 14.8% and 18.8%, respectively, after application of 50% fluazinam. The content of organic matter increased by 76.8% after treatment with 50% tebuconazole. 50% of tebuconazole had the most significant effect on total nitrogen, available phosphorus and available potassium, and the highest increase of total nitrogen was 77.8%. The maximum available phosphorus increased by 1.3 times while the content of available potassium increased by 2.1 times. The effects of fungicides on the activities of sucrase, urease and acid phosphatase in jasmine rhizosphere soil were significant. The sucrase activity was significantly decreased by 92.8% after treated with 50% fluazinam. The urease activity decreased significantly after spraying 50% tebuconazole, which was 41.9% lower than that before spraying. At the same time, the acid phosphatase activity showed increasing trends after treated with fungicides. To compare the carbon source metabolic diversity of soil microbial community after application of the fungicides found that the value of average well color development (AWCD) in CK was the lowest. After the treatment of the two fungicides, AWCD decreased compared with that before spraying, among which the inhibitory effect of tebuconazole on AWCD was the most significant. In general, the Shannon diversity index (H) and evenness index (U) of soil microorganisms showed a decreasing trend with fungicide treatment. However, there was no significant difference in the dominance index (D) among all treatments. Soil microbial community had a certain inhibitory effect on the metabolic diversity of six kinds of carbon sources after fungicides application. In addition, principal component analysis of soil microbial community carbon source metabolic diversity revealed that amino acids and carbohydrates had similar utilization rates. In addition, polymers and carboxylic acids were similar. This study analyzed and compared the effects of fluazinam and tebuconazole on the microbial characteristics and carbon source metabolic diversity in jasmine rhizosphere soil, and the results would provide a scientific basis for evaluating the ecological function in rhizosphere soil microorganisms of jasmines under the influence of two fungicides.