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Genetic Diversity Analysis of Basil (Ocimum basilicum) Germplasm Resources Based on Plant Traits and Volatile Components
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Yajiao ZHANG1, Fulai YU2, Chao YUAN2, Xiaolu CHEN2, Xiaoli XIE2, Kai WANG2, Mei HUANG2, Zhenxia CHEN2, Lingliang GUAN2, *, Songbi CHEN2, *
Chinese Journal of Tropical Crops | 2024, 45(4) : 825 - 836
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Chinese Journal of Tropical Crops | 2024, 45(4): 825-836
Post-harvest Treatment & Quality Safety
Genetic Diversity Analysis of Basil (Ocimum basilicum) Germplasm Resources Based on Plant Traits and Volatile Components
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Yajiao ZHANG1, Fulai YU2, Chao YUAN2, Xiaolu CHEN2, Xiaoli XIE2, Kai WANG2, Mei HUANG2, Zhenxia CHEN2, Lingliang GUAN2, *, Songbi CHEN2, *
Affiliations
  • 1.College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163000, China
  • 2.Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Biology and Cultivation of Chinese Medicinal Materials, Ministry of Agriculture and Rural Affairs (Trial operation) / Hainan Provincial Engineering Research Center for Tropical Medicinal Plants, Haikou, Hainan 571101, China
Published: 2024-04-25 doi: 10.3969/j.issn.1000-2561.2024.04.018
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The study was aimed to analyze the genetic diversity of the phenotypic traits and chemotypes of different Ocimum basilicum germplasm resources, to provide theoretical reference for the innovative utilization of basil germplasm resources and variety selection. The botanical traits of 15 basil germplasm resources and the volatile components of the leaves investigated and determined using gas chromatography-mass spectrometry (GC-MS), together with the relative odour activity values (ROAV). At the same time, principal component analysis and cluster analysis were carried out on the tested germplasms. The 15 basil germplasms differed significantly in plant height, plant width, leaf length, leaf width and stem thickness, except for the number of branches. 104 volatile compounds were identified, including 37 alkanes, 32 terpenes, 8 esters, 5 phenols, 4 alcohols, 3 aldehydes, 1 ether, and 14 other compounds, of which there are two types of common components, 2,4-di-tert-butylphenol and heptadecane. According to the ROAV values obtained from the enquiries and calculations, the key aroma substances were found to be eugenol, linalool, eucalyptol, cis-citral, methyl cinnamate, 4-allyl anisole, etc. The principal component analysis and cluster analysis of the volatile substances the germplasms could be classified into five groups, namely, Thai basil, Anise basil, Siam queen basil and Sweet basil were grouped together, Jiu Cengta basil, Genovese basil, Red rubin basil, Purple dark opal basil and Purple ruffles basil were grouped together, Clove basil and Lemon basil were grouped together, Tuscany basil, Pluto basil and Italian large leaf basil were grouped together, Cinnamon basil was grouped one category. The phenotypic trait cluster analysis also classified basil into five groups, with Pluto basil into a group, Clove basil, Anise basil and Purple dark opal basil into a group, Thai basil into a group, Italian large leaf basill and Cinnamon basil into a group. The remaining eight germplasm were clustered into a group. The 15 germplasms have significant differences in botanical characters and volatile components, and are rich in genetic diversity, which could be targeted for breeding of basil-specific germplasms.

Ocimum basilicum  /  botanical traits  /  volatile components  /  principal component analysis  /  cluster analysis
Yajiao ZHANG, Fulai YU, Chao YUAN, Xiaolu CHEN, Xiaoli XIE, Kai WANG, Mei HUANG, Zhenxia CHEN, Lingliang GUAN, Songbi CHEN. Genetic Diversity Analysis of Basil (Ocimum basilicum) Germplasm Resources Based on Plant Traits and Volatile Components[J]. Chinese Journal of Tropical Crops, 2024 , 45 (4) : 825 -836 . DOI: 10.3969/j.issn.1000-2561.2024.04.018
Year 2024 volume 45 Issue 4
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doi: 10.3969/j.issn.1000-2561.2024.04.018
  • Receive Date:2022-10-17
  • Online Date:2026-06-23
  • Published:2024-04-25
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History
  • Received:2022-10-17
  • Revised:2022-10-28
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Affiliations
    1.College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163000, China
    2.Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Biology and Cultivation of Chinese Medicinal Materials, Ministry of Agriculture and Rural Affairs (Trial operation) / Hainan Provincial Engineering Research Center for Tropical Medicinal Plants, Haikou, Hainan 571101, China
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表12种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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