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Banana classification is a difficult problem, using morphological features to classify is unable to meet the needs. Molecular marker technology has been gradually applied to population identification and classification, genetic relationship analysis and genetic diversity research of banana varieties (lines). In this paper, SRAP molecular marker technology was used to analyze the genetic relationship of 89 banana germplasm resources. Using 10 pairs of positive and negative primers to form 100 pairs of primer combinations, and 13 pairs of SRAP primers with easy identification, clear band type and high polymorphism were screened out. 170 identifiable bands were amplified by the 13 pairs of primers, with an average of 13.08 bands per pair of primers, of which 140 bands showed polymorphism, with a polymorphism ratio of 79.00%. The similarity coefficient of 89 banana germplasm resources varied from 0.241 to 1.000, and the genetic diversity was very rich. The similarity coefficient of the same type of wild banana from the same place or different places was high. When the similarity coefficient was 0.49, the 89 banana resources could be divided into six groups. The first major category were AAA group Xiangyajiao, AA group Gongjiao, AAB group Longyajiao and AA group wild banana (AAA, AA, AAB). The second major category were ABB group Fenjiao, ABB group Fendajiao and BB group wild banana (ABB, BB). The third major category were Dajiao and a banana wild relative Musa itinerans. M coccinea, Musella lasiocarpa, Heliconia belonged respectively to the fourth, fifth and sixth category. The results are basically consistent with the current classical classification conclusion. Only some banana germplasm have different classification status, indicating that it is feasible to use SRAP markers to classify banana germplasm resources. At the same time, the cultivated Xiangya banana, Gongya banana and Longya banana have the same ancestry, and are close to the wild banana of AA genotype, while the wild banana of BB genotype is close to the pink banana, the pink banana and the wild banana of BB genotype, and the banana germplasm resources of different genomes are gathered together, which showing that the origin of banana germplasm resources affects the genetic relationship of offspring, and a single classification method cannot completely distinguish the banana germplasm resources. In addition, the genetic relationship between plantain and plantain is relatively close, but it is impossible to distinguish whether plantain contains A and B genomes, so the genotype and genetic background of plantain need further study.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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