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Phytosulfokine (PSK) is a small peptide hormone unique to higher plants, widely involved in biological processes such as plant growth and development, division and differentiation, biotic and abiotic stress. PSK receptor (PSKR) is a direct receptor for PSK and is crucial for PSK signal transduction. In the present study, HbPSKR2 was cloned from rubber tree by RT-PCR (reverse transcription-polymerase chain reaction), and its bioinformatics, gene expression pattern, interacted proteins screening and identification were also analyzed. Results showed that the open reading frame of reading frame of HbPSKR2 had a total length of 3159 bp and encoded 1052 amino acids with molecular weight 114.84 kDa and theoretical isoelectric point 6.34. Domain analysis showed that HbPSKR2 was a typical trans-membrane protein. The first 640 amino acids were the antenna structures composed of leucine rich repeat (LRR), the 692 to 714 amino acids were transmembrane domains, and the 765 to 1052 amino acids were kinase domains. Multiple comparisons were conducted on the membrane kinase domain of HbPSKR2 and the membrane kinase domain of PSKR homologous proteins in Arabidopsis and rice. The results showed the existence of conserved sites such as ATP binding site, CaM binding site, Activation segment, GC Centre. Expression pattern analysis showed that HbPSKR2 was highly expressed in the cambium region of rubber tree. The expression level of HbPSKR2 was significantly increased at the early stage of coronatine (COR) treatment. Twelve candidate proteins interacted with HbPSKR2 were screened by yeast two hybrid technology, and the interaction between two protein kinases (HbPBL8 and HbPIX13) and HbPSKR2 was further verified by luciferase complementary imaging. The strong fluorescence signal was observed by co-transformation HbPSKR2-nLUC/HbPBL8-cLUC and HbPSKR2-nLUC/HbPIX13-cLUC into tobacco, suggesting the interaction between HbPSKR2-HbPBL8 and HbPSKR2- HbPIX13 in vivo. The cloning of HbPSKR2 and identification of HbPSKR2 interacted protein would provide new insights into the molecular mechanism of laticifer differentiation in rubber tree.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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