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In order to enhance the antifungal activity of Streptomyces samsunensis 17-7 strain and effectively utilize its secondary metabolites, this study focused on optimizing the fermentation medium formula. Orthogonal and single factor experiments were conducted based on the original medium, with Phellinus noxious as the indicator. The optimal formulation for producing active substances from the strain was determined to include 25 g/L soy powder, 10 g/L corn meal,10 g/L glucose, 2 g/L yeast extract, 1 g/L K2HPO4, 0.5 g/L NaCl, and 0.5 g/L CaCO3. Following a 500 times dilution, the relative antifungal activity of the fermentation solution increased to 51.37%, a 17.66% improvement compared to the previous optimization, demonstrating a broad spectrum of antifungal activity. The stability of the antifungal substance was evaluated in response to various conditions such as temperature, pH, protease, UV-irradiation, and storage. Results indicated that the fermentation broth of strain 17-7 exhibited high temperature resistance, a significant decrease in antifungal activity after one hour of UV irradiation, but retained its activity after long-term exposure to UV light. Furthermore, the broth demonstrated good acid-base tolerance, effective antibacterial activity in the pH range of 3-10, and resistance to trypsin and pepsin. The fermentation broth of strain 17-7 maintained its antifungal activity for 120 days at both 4 ℃ and room temperature (25 ℃). This study on the stability and storage conditions of strain 17-7’s fermentation broth offers valuable data for potential future applications of the strain as a biocontrol agent.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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