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Detection of Papaya PRSV Virus Using ELISA and qRT-PCR Techniques
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Xiuju XIE1, Qiyu XIA1, Shanshan HUO1, Xiaoxi DU1, Xianjun MAI2, Yuliang ZHANG1, Anping GUO1, Feng LI3, Xiangyi KONG2, **, Hui ZHAO1, **
Chinese Journal of Tropical Crops | 2024, 45(12) : 2534 - 2541
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Chinese Journal of Tropical Crops | 2024, 45(12): 2534-2541
• Omics & Biotechnology •
Detection of Papaya PRSV Virus Using ELISA and qRT-PCR Techniques
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Xiuju XIE1, Qiyu XIA1, Shanshan HUO1, Xiaoxi DU1, Xianjun MAI2, Yuliang ZHANG1, Anping GUO1, Feng LI3, Xiangyi KONG2, **, Hui ZHAO1, **
Affiliations
  • 1.Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences / Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences / Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions, Sanya, Hainan 572024, China
  • 2.Sanya Academy of Tropical Agriculture, Sanya, Hainan 572022, China
  • 3.Bellagen Biotechnology Co., Ltd., Jinan, Shandong 250300, China
Published: 2024-12-25 doi: 10.3969/j.issn.1000-2561.2024.12.005
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Papaya ringspot virus (PRSV) is one of the most serious diseases in papaya production, with high incidence rate, rapid transmission and serious harm. To detect papaya plants infected with PRSV in a timely manner, this study established methods for detecting papaya plants infected with PRSV using enzyme-linked immunosorbent assay (ELISA) and fluorescence quantitative reverse transcription PCR (qRT-PCR). The two methods were used to detect the PRSV content of multiple transgenic and non transgenic papaya plants, and the results were compared. The results showed that the standard curve established using PRSV peptide antigen as the standard and antibodies prepared from it had good fitting, and could be used for ELISA detection of PRSV; The reference gene Cpa03g018830 selected in qRT-PCR method was stably expressed at different growth stages of papaya and could be used as a reference gene for PRSV content determination; The detection results of PRSV content in multiple transgenic and non transgenic papaya plants using ELISA and qRT-PCR methods were basically consistent, indicating that both methods can be used for the detection of PRSV content in papaya plants. By using thee two detection methods, papaya plants infected with PRSV can be detected and eradicated in a timely manner, effectively preventing and controlling the spread of PRSV.

PRSV  /  ELISA  /  qRT-PCR
Xiuju XIE, Qiyu XIA, Shanshan HUO, Xiaoxi DU, Xianjun MAI, Yuliang ZHANG, Anping GUO, Feng LI, Xiangyi KONG, Hui ZHAO. Detection of Papaya PRSV Virus Using ELISA and qRT-PCR Techniques[J]. Chinese Journal of Tropical Crops, 2024 , 45 (12) : 2534 -2541 . DOI: 10.3969/j.issn.1000-2561.2024.12.005
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Year 2024 volume 45 Issue 12
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Article Info
doi: 10.3969/j.issn.1000-2561.2024.12.005
  • Receive Date：2024-05-10
  • Online Date：2026-06-23
  • Published：2024-12-25
Article Data
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History
  • Received：2024-05-10
  • Revised：2024-07-10
Funding
Affiliations
    1.Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences / Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences / Hainan Key Laboratory for Biosafety Monitoring and Molecular Breeding in Off-Season Reproduction Regions, Sanya, Hainan 572024, China
    2.Sanya Academy of Tropical Agriculture, Sanya, Hainan 572022, China
    3.Bellagen Biotechnology Co., Ltd., Jinan, Shandong 250300, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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