Using the robusta coffee Reyan 1 as the scion and liberica coffee Chali 5 as the rootstock, grafting was carried out using the inarching method to investigate the effect of interspecies grafting on the growth of coffee grafted seedlings. Two sets of inarching methods were set up (cutting off the top of the rootstock first and then grafting, and grafting first and then cut off the top of the rootstock after surviving), and then transplanted into three types of soil (clay, loam, and sandy soil) and four different coffee continuous cropping years (0, 5, 10, 20 a) soil, and the robusta coffee self grafted seedlings were used as CK to study the effect of interspecific inarching on the survival rate, growth index, chlorophyll content, and growth and degradation of grafted seedlings in the different soil types and in the different coffee continuous cropping years soil. Results showed that grafting at the same stage of scion, the survival rate of F2 treatment (grafted seedlings with preserved aboveground parts of the rootstock) was significantly higher than that of F1 treatment (grafted seedlings without preserved aboveground parts of the rootstock). Using the same method, grafting at the “Butterfly” stage of the scion, the survival rate of grafting seeding was significantly reduced compared to grafting at the “soldier” stage. Different grafting methods could significantly affect the survival rate of coffee grafted seedlings, and the younger age of the scion seedlings was beneficial for improving the survival rate of grafted seedlings. The treatment of F1 increased the root system, root to shoot ratio, and promoted the increase of aboveground biomass, resulting in faster plant growth. In the early stage of F2 treatment, the biomass of the aboveground part was high, which promoted the growth of the underground part. Cutting off the top of the rootstock after surviving relatively reduced the biomass of the aboveground part, inhibited the growth of the root system, which reduced the biomass of the root system. Different soil types had a significant impact on the growth of the root system of grafted seedlings. In clay, the root system of robusta coffee seedlings was more developed than that of liberica coffee seedlings, while in sandy soil, the root system of liberica coffee seedlings was more developed than that of robusta coffee seedlings. As the grafted seedlings grew, if the root system of robusta coffee seeds was not cut off in time after grafting and surviving, the root system of liberica coffee seedings would slowly deteriorate with growth. In soils with different coffee continuous cropping years, the survival rate of interspecific inarching seedlings was 100%, significantly improving the survival rate, effectively alleviating continuous cropping obstacles, improving survival rate, and restoring aboveground growth. On the 2–3 days after grafting, the parenchyma cell in the cortex began to coalescent. On the 4th day, callus tissue could be seen between the rootstock and scion. On the 6–8 days, 2/3 of the stem segments between the rootstock and scion had already healed, and healing was evident on the 8–10 days. Using liberica coffee as rootstocks and robusta coffee as scions, the method of cutting off the top of liberica coffee seeding first and then inarching during the “soldier stage” of the scion can obtain vigorous interspecific grafting seedlings, which can effectively alleviate soil continuous cropping obstacles and improve coffee growth.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |