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Lysophosphatidyl acyltransferase (LPAT), a key enzyme which controls the production of lysophosphatidic into phosphatidic, plays an important role in phospholipid biosynthesis in plants. Hevea brasiliensis is an important source for the production of natural rubber, which has an irreplaceable role in the fields of national defense, medical treatment, high-end products, and has high social and economic value. Cloning and analysis of HbLPAT2 in H. brasiliensis would provide scientific basis for further exploration of its phospholipid biosynthesis function and the effect of phospholipid on nature rubber. In this study HbLPAT2 was cloned from the latex of the H. brasiliensis by molecular biology techniques, and the bioinformatics analysis, expression analysis and subcellular localization analysis were performed. In this study, two key genes responsible for phospholipid biosynthesis in the HbLPAT gene family were successfully cloned from the latex of the H. brasiliensis, named HbLPAT2a and HbLPAT2b, the full length coding sequence of HbLPAT2a and HbLPAT2b genes was 1161 bp and 939 bp, encoding 386 and 312 amino acids. Both HbLPAT2a and HbLPAT2b contained a PLN02380 lysopyatidyl acyltransferase functional domain and contained a highly conserved NH(X)4D motif; HbLPAT2a protein had 3 transmembrane domains, and HbLPAT2b protein had 1 transmembrane domain, both of which had no signal peptide, were hydrophilic proteins, and contained multiple phosphorylation sites. The subcellular localization analysis showed that both HbLPAT2a and HbLPAT2b proteins were located in the endoplasmic reticulum. In different organs, the highest expression level of HbLPAT2a and HbLPAT2b was found in seeds, suggesting that they have a lipid biosynthesis function. However, in the latex of H. brasiliensis, the first and second expression level of whole HbLPAT gene family were HbLPAT2a and HbLPA2b. The result is helpful to understanding that HbLPAT2a and HbLPAT2b play an important role in the phospholipid biosynthetic pathway in latex of H. brasiliensis and to providing a scientific basis for genetic improvement of latex quality of H. brasiliensis.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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