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Kadsura coccinea (Lem.) A. C. Smith, a traditional medicinal plant widely cultivated in South China, has experienced significant yield and quality reductions due to increasing disease incidence in recent years. This study aimed to identify the causal agent of leaf blight disease in K. coccinea and evaluate fungicide efficacy to establish a scientific foundation for pathogen identification and field management. Pathogens were isolated from symptomatic K. coccinea samples collected in Shaoguan, Guangdong and showing browning and necrosis of leaf and stem tips, using tissue isolation and purification methods. Pathogen was clarified by comprehensive identification combined morphological characterization with multi-locus phylogenetic analysis (ITS, rpb2, LSU and tub2). Pathogenicity was confirmed through Koch's postulates, while the indoor toxicity of seven fungicides to the pathogen was determined via mycelial growth inhibition assays. Isolate SGXFD, obtained from the edges of necrotic leaf tissues, was confirmed as the etiological agent through pathogenicity tests. Polyphasic identification classified the pathogen as Didymella segeticola. Fungicide screening revealed dimetachlone as the most effective inhibitor (EC50=1.8210 mg/L), followed by prochloraz (EC50=3.4460 mg/L) and propiconazole (EC50=3.7391 mg/L). Moderate inhibition was observed with difenoconazole (EC50=11.3202 mg/L), tebuconazole (EC50=45.0019 mg/L), and mancozeb (EC50=51.9597 mg/L). Carbendazim demonstrated limited efficacy (<50% inhibition) across tested concentrations (25.0, 50.0, 100.0, 200.0, 400.0 mg/L). This study establishes D. segeticola as the causative agent of K. coccinea leaf blight. Dimetachlone, prochloraz, and propiconazole exhibit significant antifungal activity against this pathogen, recommending the consideration for field application in disease management programs.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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