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Carbonic anhydrase (CA) plays an important role in the photosynthesis of plants and response to stress. Cassava CA gene was cloned using the cDNA of SC124 as template. The CDS length of MeCA was 1008 nt, encoding 336 amino acids. The homology was 99.70% between the cloned sequence and Manes.15G167500.1 in the database. The evolutionary tree resulted that MeCA belonged to the same branch as the AtβCA subfamily of Arabidopsis. The sequence similarity between MeCA and AtβCA1 protein reached 73.13% and contained identical motif. MeCA gene expression was the highest in functional leaves, followed in young leaves, significantly higher in fibrous roots and stems, and significantly down-regulated in shading stressed cassava leaves. In addition, the gene showed significant upregulated expression in the initial stage, and then it dropped to the control level when cassava leaves were treated with low temperature. This study created transgenic cassava with overexpression of MeCA gene, and observed that MeCA protein was localized in chloroplasts, and the contents of various chlorophyll were significantly higher in the leaves of transgenic plants than that of control. The results of yeast two-hybrid point-to-point experiments showed that MeCA protein interacted with MeH1.2. The result indicated that MeCA gene could respond to light and low temperature stresses, and maight participate in plant response to stress by affecting plant growth interacting with MeH1.2 proteins. This study would provide a new gene resource for genetic improvement of cassava resistance.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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