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Tea tree oil (TTO) chitosan emulsion was prepared by ultra-high pressure homogenization method with natural TTO and chitosan as raw materials, Tween-80 as emulsifying agent, and ethanol as co-emulsifying agent. The preparation conditions were optimized by response surface test. The storage stability of TTO chitosan emulsion at different temperatures was investigated, and its antioxidant activity and mildew proof properties were evaluated. The optimal preparation conditions of the emulsion were as follows, the homogenization pressure 160 MPa, the mass fraction of tea tree oil 12%, and the mass fraction of compound emulsifier 4%. Under the conditions, the particle size of the emulsion was 99.24 nm. When stored at 4, 25 and 50 ℃ for 28 days, the particle size remained within 164 nm, the Zeta potential all exceeded 39 mV, and the PDI remained within 0.53, indicating that the storage stability of the emulsion was good. The IC50 value of DPPH radical and OH radical of the emulsion was 22.82 μL/mL and 18.75 μL/mL, respectively, indicating that the emulsion had good antioxidant activity. The mildew proof test showed that the mildew proof ability of bamboo treated with TTO chitosan emulsion was higher than that of untreated bamboo. When the treatment concentration was 80 μL/mL, the antifungal effect was significant.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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