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Prokaryotic Expression and Antiserum Preparation of the Movement Protein Gene of Cucumber Green Mosaic Virus
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Chunmei REN, Zhaobang CHENG, Liu YANG, Shuo LI, Haitao WANG, Fang LU, Yinghua JI*
Chinese Journal of Tropical Crops | 2025, 46(9) : 2181 - 2189
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Chinese Journal of Tropical Crops | 2025, 46(9): 2181-2189
• Plant Protection & Bio-safety •
Prokaryotic Expression and Antiserum Preparation of the Movement Protein Gene of Cucumber Green Mosaic Virus
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Chunmei REN, Zhaobang CHENG, Liu YANG, Shuo LI, Haitao WANG, Fang LU, Yinghua JI*
Affiliations
  • Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, China
Published: 2025-09-25 doi: 10.3969/j.issn.1000-2561.2025.09.016
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Cucumber green mottle mosaic virus (CGMMV) is one of the important plant viruses that harm melon crops, and its movement protein (MP) plays a key role in virus transmission and pathogenicity. This study cloned the MP gene of CGMMV through RT-PCR, constructed the prokaryotic expression vector pET-32a-MP, and successfully induced the expression of a recombinant MP fusion protein with a molecular weight of approximately 48 kDa in Escherichia coli BL21 (DE3). Purification of high purity protein (purity degree≥80%) using nickel column affinity chromatography was used to prepare polyclonal antibodies against the New Southwest White Rabbit. Western blot and ELISA analysis showed that the titer of the anti serum reached 409 600, and it could specifically recognize the MP protein in CGMMV infected leaves, without cross reactivity with other viruses such as Tobacco mosaic virus (TMV) and Watermelon mosaic virus (WMV). The study would provide important tools for rapid virus detection, immunohistochemistry, and protein function research, which is of great significance for ensuring the safe production of melon crops.

Cucumber green mottled mosaic virus  /  sports protein  /  prokaryotic expression  /  antiserum
Chunmei REN, Zhaobang CHENG, Liu YANG, Shuo LI, Haitao WANG, Fang LU, Yinghua JI. Prokaryotic Expression and Antiserum Preparation of the Movement Protein Gene of Cucumber Green Mosaic Virus[J]. Chinese Journal of Tropical Crops, 2025 , 46 (9) : 2181 -2189 . DOI: 10.3969/j.issn.1000-2561.2025.09.016
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Article Info
doi: 10.3969/j.issn.1000-2561.2025.09.016
  • Receive Date：2025-03-18
  • Online Date：2026-03-07
  • Published：2025-09-25
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  • Received：2025-03-18
  • Accepted：2025-05-14
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    Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, Jiangsu 210014, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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