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Effects of Dehydration Duration on Physiological Parameters of Rubber Tree Anther Callus Tissue During Cryopreservation
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Quannan ZHOU1, Xin JI4, Yinle WU4, Ji LI1, Xianfeng YANG1, 2, 3, Rizhi WU1, Tiandai HUANG1, 2, 3, *
Chinese Journal of Tropical Crops | 2025, 46(9) : 2127 - 2134
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Chinese Journal of Tropical Crops | 2025, 46(9): 2127-2134
• Plant Cultivation, Physiology & Biochemistry •
Effects of Dehydration Duration on Physiological Parameters of Rubber Tree Anther Callus Tissue During Cryopreservation
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Quannan ZHOU1, Xin JI4, Yinle WU4, Ji LI1, Xianfeng YANG1, 2, 3, Rizhi WU1, Tiandai HUANG1, 2, 3, *
Affiliations
  • 1.Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences/Haikou Key Laboratory of Tropical Plant Seedling Innovation/Key Laboratory of Rubber Tree Biology and Genetic Resources Utilization, Ministry of Agriculture and Rural Affairs/Hainan Provincial Key Laboratory of Tropical Crop Cultivation Physiology/Cultivation Base of State Key Laboratory Jointly Built by the Province and Ministry, Haikou, Hainan 571101, China
  • 2.Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya, Hainan 572025, China
  • 3.National Key Laboratory for Tropical Crop Breeding, Sanya, Hainan 572025, China
  • 4.College of Tropical Crops, Yunnan Agricultural University, Pu'er, Yunnan 665099, China
Published: 2025-09-25 doi: 10.3969/j.issn.1000-2561.2025.09.010
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Cryopreservation can reduce the risk of genetic variation in the long-term subculture of rubber tree anther callus tissue, and is an effective method for long-term preservation of callus tissues. To evaluate the effects of dehydration duration on physiological parameters during cryopreservation, rubber tree anther calli were treated with plant vitrification solution PVS2 for 0, 10, 20 and 40 min and then cryopreserved for 24 h in this study. Physiological parameters related to stress resistance were analyzed, including soluble protein content, malondialdehyde (MDA) content, and activities of catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD) during cryopreservation. Dehydration duration had a significant effect on soluble protein content, MDA content, SOD and POD activities in callus tissues after cryopreservation, while showing no significant impact on CAT activity. All physiological parameters (except MDA) exhibited higher values after cryopreservation compared to pre-preservation levels across all dehydration durations. After 40 min of dehydration and cryopreservation, the soluble protein content, CAT, SOD and POD activities of anther callus reached the maximum value, which was 129.63 μg/mL, 627.30 U/g, 290.38 U/g and 25 643.33 U/g, with the lowest MDA content of 41.31 nmol/g. The findings indicate that dehydration for 40 min significantly enhances water retention capacity and antioxidant level in cryopreserved callus cells. Post-thawing viability tests revealed that 40 min dehydrated callus maintained over 70% survival rate and could induce fragile embryogenic callus formation with an average induction rate of 13.33%. This study establishes a physiological foundation for the regeneration of cryopreserved rubber tree anther callus.

rubber tree  /  anther callus tissue  /  cryopreservation  /  dehydration duration  /  physiological parameters  /  viability
Quannan ZHOU, Xin JI, Yinle WU, Ji LI, Xianfeng YANG, Rizhi WU, Tiandai HUANG. Effects of Dehydration Duration on Physiological Parameters of Rubber Tree Anther Callus Tissue During Cryopreservation[J]. Chinese Journal of Tropical Crops, 2025 , 46 (9) : 2127 -2134 . DOI: 10.3969/j.issn.1000-2561.2025.09.010
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Year 2025 volume 46 Issue 9
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Article Info
doi: 10.3969/j.issn.1000-2561.2025.09.010
  • Receive Date：2025-04-08
  • Online Date：2026-03-07
  • Published：2025-09-25
Article Data
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History
  • Received：2025-04-08
  • Accepted：2025-06-03
Funding
Affiliations
    1.Rubber Research Institute, Chinese Academy of Tropical Agricultural Sciences/Haikou Key Laboratory of Tropical Plant Seedling Innovation/Key Laboratory of Rubber Tree Biology and Genetic Resources Utilization, Ministry of Agriculture and Rural Affairs/Hainan Provincial Key Laboratory of Tropical Crop Cultivation Physiology/Cultivation Base of State Key Laboratory Jointly Built by the Province and Ministry, Haikou, Hainan 571101, China
    2.Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya, Hainan 572025, China
    3.National Key Laboratory for Tropical Crop Breeding, Sanya, Hainan 572025, China
    4.College of Tropical Crops, Yunnan Agricultural University, Pu'er, Yunnan 665099, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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