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Lignin serves a crucial defensive function in cassava's resistance to harmful organisms. Among the genes involved in lignin biosynthesis, shikimate hydroxycinnamoyl transferase (HCT) and caffeoyl coenzyme A-O-methyltransferase (CCoAOMT) are of particular importance. Yet, their specific roles in cassava's resistance to the insect pest P. marginatus remain to be fully elucidated. To elucidate the anti-insect defense mechanisms of cassava against P. marginatus, we conducted comparative studies using insect-resistant cultivar C1115 and susceptible cultivar KU50. Life table analysis revealed significant cultivar-dependent effects on pest developmental biology. Specifically, C1115-fed females exhibited significantly prolonged developmental duration (38.50 days in C1115 vs. 28.86 days in KU50), significantly reduced fecundity (221 eggs in C1115 female vs. 331 eggs in KU50 female), and significantly shortened adult longevity (4.30 days in C1115 vs. 8.83 days in KU50). Notably, population growth parameters demonstrated significant comprehensive suppression in C1115 cohorts: net reproductive rate (R0=224.13 in C1115 vs. 330.67 in KU50), intrinsic growth rate (rm=0.10 d‒1 in C1115 vs. 0.12 d‒1 in KU50), and finite growth rate (λ=1.11 d‒1 in C1115 vs. 1.12 d‒1 in KU50). Furthermore, extended generation time (T=50.25 in C1115 vs. 49.98 days in KU50) and doubling time (Dt=6.44 in C1115 vs. 5.97 days in KU50) were observed, indicating multilevel inhibition of pest population dynamics by C1115. Molecular characterization showed progressive downregulation of lignin biosynthesis genes HCT and CCoAOMT post-infestation in C1115. Although both cultivars exhibited suppression patterns, C1115 maintained significantly lower expression levels (52.99%, 66.81% of KU50) and enzymatic activities (74.42%, 73.91% of KU50) across timepoints. Using Spearman correlation analysis, it was found that both HCT and CCoAOMT gene expression levels and the activity of their encoded enzymes were negatively correlated with cassava resistance to P. marginatus. This suggests that the downregulation of these two genes may affect lignin accumulation in cassava leaves and the level of insect resistance defense responses. This study has laid a solid preliminary work foundation for in-depth analysis of the molecular mechanisms by which lignin regulates cassava's defense responses against P. marginatus
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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