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Sri Lankan cassava mosaic disease, caused by Sri Lankan cassava mosaic virus (SLCMV), is a recently emerging dangerous disease in China. Existing detection methods of SLCMV are constrained by low sensitivity and poor efficiency, impeding related research and applications. Primers and probes were designed according to the gene sequences of the SLCMV, and a positive plasmid standard was prepared. The TaqMan fluorescence quantitative detection technology for SLCMV was established, and its application effect was verified. The method only generated specific fluorescence signals for SLCMV DNA samples, and the minimum detectable amount of the positive plasmid standard was 4.5×101 copies/μL. The standard curve showed that there was a good linear relationship between the Ct value and the logarithm of the copy number. The slope of the curve was –3.1312, the correlation coefficient R2 was 0.9969, the amplification efficiency (E) was 97.9%, and the equation of the standard curve was y=–3.1312x+34.599. Using this technology to detect the tested samples from two cassava plantations in Guangxi and Fujian, the positive detection rate of leaves was 95.45% and 78.57%, respectively, and the minimum detectable copy number was 1.45×105 copies/g. The virus-carrying rate of Bemisia tabaci in the field was 86%, and the minimum virus-carrying amount was 9.42×104 copies/B. tabaci. This technology has good sensitivity, specificity, and repeatability, and could provide effective technical support for the monitoring and control work such as field identification, early diagnosis, and evaluation of virus-free stem cuttings of the disease.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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