A comprehensive evaluation of agronomic traits of 70 varieties of fresh food tomato germplasms was conducted. Genetic diversity analysis of 28 morphological traits of the germplasms was carried out using methods such as variance analysis, principal component analysis, cluster analysis and calculation of comprehensive evaluation scores. The analysis results of genetic variability of 13 quantitative traits indicated that the variation degree of the germplasms was relatively high and the variation was rich. The variation range was 21.68%‒99.08%, and the genetic diversity index was relatively high, suggesting that the phenotypic diversity of germplasms was relatively rich and there was a significant degree of variation among the traits, indicating excellent selection potential. Among these, the traits such as single fruit weight, number of chambers, sugar-acid ratio, and transverse diameter exhibit relatively high genetic variation. The correlation analysis results showed that the traits significantly corelated with fruit firmness were the greateast in number, and fruit firmness were extremely significantly positively correlated with fruit surface ridges, fleshiness, single fruit weight, number of chambers, flesh thickness, and acidity. Sugar content was significantly negatively correlated with fruit color before maturity, and it was extremely significantly negatively correlated with fruit surface ridges, single fruit weight, number of chambers, and flesh thickness, and significantly positively correlated with plant height, and extremely significantly positively correlated with fruit shape index and soluble solids content. Single fruit weight was significantly positively correlated with leaf type, significantly negatively correlated with fruit peel color, extremely significantly positively correlated with flower sequence type and fruit surface ridges, and extremely significantly negatively correlated with fruit top shape and fruit shape. The principal component analysis results indicated that the 28 phenotypic traits could be classified into nine factors. The eigenvalues were all above 1.0, and the cumulative contribution rate reached 78.143%, which could reflect most of the information of all indicators and highlight the basic characteristics of the germplasms. Cluster analysis further divided the germplasms into five groups. The first and fourth groups had smaller tomato fruits and stronger edible properties, which could be important materials for cultivating cherry tomato varieties with good taste and high quality. The fifth group had higher hardness and larger fruits, and could be used to cultivate economic tomato varieties suitable for long-distance transportation. The comprehensive evaluation results showed that the top five germplasms were XH20-48, XH20-35, XH20-37, XH20-34 and XH20-30. In conclusion, this research achievement is dedicated to discovering and exploring the excellent tomato germplasm resources retained by the research group, laying a foundation for the next step of creating core parents and breeding new high-quality tomato varieties with characteristics unique to Xinjiang.