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The effects of mixed silage of Stylosanthes Guianensis Reyan No. 2 and Pennisetum purpureum × P. glaucum cv. Reyan No. 4 on fermentation quality and nutrient composition were studied to screen out the appropriate proportion of mixed silage, and provide theoretical basis for the preparation of high-quality silage. The experiment was divided into 4 groups. The mixed silage ratios of Reyan No. 4 and Reyan No. 2 were 100∶0 (CK), 85∶15 (A1), 70∶30 (A2) and 55∶45 (A3), respectively. Ensilage was unsealed for 30 days and sampled for detection. A2 had the best sensory evaluation. With the increase of the ratio of Reyan No. 2, pH and NH3-N/TN values gradually increased, and there were significant differences between A1, A2, A3 and CK (P<0.05). The lactic acid (LA) content of A3 was significantly lower than that of CK (P<0.05). The lactic acid/acetic acid (LA/AA) value was greater than 2.6 in all experimental groups. Butyric acid (BA) in A2 was significantly lower than that in other groups (P<0.05). With the proportion of Reyan No.2 increasing, the content of crude protein (CP), dry matter (DM) and ether extract (EE) in all groups increased, and were significantly higher than those of CK (P<0.05). Water-soluble carbohydrate (WSC) content showed an increasing trend, and there was no significant difference among groups (P>0.05). Neutral detergent fiber (NDF) decreased gradually, and there was no significant difference among all groups (P>0.05). Acid detergent fiber (ADF) in all groups was significantly decreased (P<0.05). The ash content of each group decreased, and there was no significant difference among A1, A2 and A3 compared with CK (P>0.05). In conclusion, the mixed silage of Reyan No. 2 and Reyan No. 4 can improve the fermentation quality and nutritional value of silage. Considering the fermentation quality and nutritional value, it is suggested that A2 is suitable for silage.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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