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Phalaenopsis is the most popular Spring Festival pot flower in China. In order to accurately regulate the flowering stage and excavate the key flowering gene in the production and cultivation of Phalaenopsis, this paper studied the effects of growth regulators GA3 and 6-BA on the flowering of Phalaenopsis Big Chilli, and the PhPIF4 gene selected from transcriptome data was cloned, and its bioinformatics analysis and response to growth regulator GA3 were studied. The results showed that PhPIF4 had a typical conserved domain of the bHLH_SF super family (cl00081) homobox family. PhPIF4 of Big Chilli is closely related to Phalaenopsis equestris, followed by Cymbidium ensifolium, C, sinense and Dendrobium catenatum. The protein encoded by PhPIF4 was localized in non-chloroplast organelles. GA3 treatment increased stalk length and the maximum stalk length of the treatment group was 200 mg/L GA3. GA3 could bring forward flowering 9-11 d. 6-BA significantly increased the number of flowers, and the treatment group with the most flowering amount was 300mg/L 6-BA, but 6-BA had no significant effect on flowering time and stalk length. When the two growth regulators were mixed to spray, the effect on flowering time and flowering time of Phalaenopsis was between the two. PhPIF4 was closely related to the development of flowering, and its expression gradually increased with the development of flowering. After spraying GA3, the expression of PhPIF4 in flower buds, leaves and roots increased, and the increase was the largest in flower bud stage, suggesting that the expression of PhPIF4 might be affected and regulated by GA3, and PhPIF4 might participate in the gibberellin flowering pathway. In this paper, the effects of GA3 and 6-BA on reproductive growth of Phalaenopsis were studied, and the expression of PhPIF4 in response to GA was investigated, which would lay a foundation for revealing the role of PhPIF4 in gibberellin regulation of growth and development regulatory network.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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