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Comparison of Leaf Morphological Structure and Heat Tolerance of Six Camellia gauchowensis Chang Clones
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Huibin LIU1, 2, Zhoujun ZHU1, 2, Junru ZHAO1, 2, Yuanxian LU3, Kai WU3, Deyi YUAN1, 2, *
Chinese Journal of Tropical Crops | 2023, 44(4) : 737 - 745
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Chinese Journal of Tropical Crops | 2023, 44(4): 737-745
Plant Cultivation, Physiology & Biochemistry
Comparison of Leaf Morphological Structure and Heat Tolerance of Six Camellia gauchowensis Chang Clones
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Huibin LIU1, 2, Zhoujun ZHU1, 2, Junru ZHAO1, 2, Yuanxian LU3, Kai WU3, Deyi YUAN1, 2, *
Affiliations
  • 1.Key Laboratory of Economic Forest Cultivation and Protection, Ministry of Education, Central South University of Forestry and Technology, Changsha, Hunan 410004, China
  • 2.Key Laboratory of Economic Forest Breeding and Cultivation State Forestry Administration, Changsha, Hunan 410004, China
  • 3.Qingyuan Guzhen Tea Oil Development Co., Ltd., Qingyuan, Guangdong 511500, China
Published: 2023-04-25 doi: 10.3969/j.issn.1000-2561.2023.04.010
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The evaluation index system of heat resistance of Camellia gauchowensis Chang was studied to screen out varieties with strong heat resistance, and to provide theoretical basis for the breeding and cultivation of heat resistant varieties. Using six Camellia gauchowensis Chang clones as the test materials, 14 leaf morpholog, anatomical structure and leaf stoma related indexes were measured by image software analysis, paraffin section and nail polish blotting methods. Cluster analysis and correlation analysis were performed by SPSS software to screen out the main indicators related to heat resistance. The average membership function was calculated by EXCEL software for comprehensive evaluation of heat resistance and ranking. The leaves of C. gauchowensis Chang were composed of upper and lower epidermal cells, mesophyll tissue cells and leaf midribs. The leaf was ectopic leaves, the thickness of the upper epidermis was greater than that of the lower epidermis, the stomata was distributed in the lower epidermis, the palisade tissue was closely arranged 1-2 layers, and the spongy tissue was loosely arranged. leaf midribs were mainly composed of upper and lower epidermis and closely arranged vascular bundles. There were significant differences in leaf morphology and anatomical structure among the six clones (P<0.05). The 14 indicators were divided into four categories by systematic clustering. Leaf length, leaf width and leaf area were in the first category, leaf thickness, palisade tissue thickness, leaf structure compactness and palisade-sea ratio were in the second category, thickness of the upper epidermis, the thickness of the lower epidermis, leaf structure porosity, sponge tissue thickness and stomatal density were in the third category, and thickness of the leaf midrib and the thickness of the vascular bundle were in forth category. Leaf area, palisade tissue thickness, spongy tissue thickness and vascular bundle thickness were found to be the typical indicators of heat resistance of C. gauchowensis Chang by correlation coefficients analysis. The membership function formula was used to calculate the four typical indicators, and the comprehensive calculation and sorting were carried out according to the average membership degree. The heat resistance ranking of the six C. gauchowensis Chang clones was A16>A5>HMZ24>A11>HM19>HMZ25. The leaf indicators affecting the heat resistance of C. gauchowensis Chang are mainly leaf area, palisade tissue thickness, spongy tissue thickness and vascular bundle tissue thickness. There is a big difference in the analysis of a certain index, and the comprehensive comparative analysis through the membership function method can reflect the difference in heat resistance of Camellia gauchowensis Chang.

Camellia gauchowensis Chang  /  leaf  /  anatomical structure  /  heat resistance
Huibin LIU, Zhoujun ZHU, Junru ZHAO, Yuanxian LU, Kai WU, Deyi YUAN. Comparison of Leaf Morphological Structure and Heat Tolerance of Six Camellia gauchowensis Chang Clones[J]. Chinese Journal of Tropical Crops, 2023 , 44 (4) : 737 -745 . DOI: 10.3969/j.issn.1000-2561.2023.04.010
Year 2023 volume 44 Issue 4
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doi: 10.3969/j.issn.1000-2561.2023.04.010
  • Receive Date:2022-05-10
  • Online Date:2026-03-05
  • Published:2023-04-25
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History
  • Received:2022-05-10
  • Revised:2022-07-08
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Affiliations
    1.Key Laboratory of Economic Forest Cultivation and Protection, Ministry of Education, Central South University of Forestry and Technology, Changsha, Hunan 410004, China
    2.Key Laboratory of Economic Forest Breeding and Cultivation State Forestry Administration, Changsha, Hunan 410004, China
    3.Qingyuan Guzhen Tea Oil Development Co., Ltd., Qingyuan, Guangdong 511500, China
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表12种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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