Praxelis clematidea (Griseb.) is one of the most important invasive plants in China, which is continuously spreads with the increasingly serious nitrogen deposition in terrestrial ecosystem. The objective of the study was to reveal the responses of growth characteristics and accumulate the basic data for formulating corresponding prevention and control strategies using simulated nitrogen deposition. The growth characteristics such as root and plant length, leaf number and leaf area, root characteristics, biomass and its distribution and chlorophyll content in 45 days with four nitrogen deposition levels (control of 0, low nitrogen group of 40 kg/hm², medium nitrogen group of 60 kg/hm², high nitrogen group of 80 kg/hm²) compared with the 90 days were measured respectively. The results showed that the developmental ability was significantly improved in both growth periods (P<0.05) with the increase of nitrogen deposition level. Compared with N₀, the root length and plant height increased by 44.38% and 60.89% after N₃ culture for 90 days. The number of leaves and leaf area increased by 122.78% and 119.32%, and the growth rate of total root length, root surface area, root volume and root mean diameter was 56.04%, 42.36%, 59.38% and 61.54%. At 45 d, compared with the control group, total biomass of N₁, N₂ and N₃ increased by 37.50%, 81.25% and 106.25%, and the corresponding data at 90 d was 23.81%, 61.90% and 80.95%, respectively. Further comparison of the biomass distribution characteristics with different nitrogen concentrations at 45 d and 90 d showed that the different concentrations of nitrogen had inhibitory effects on RBR and R/S, and the inhibitory effect of low concentration nitrogen and early growth stage was stronger than that of high concentration nitrogen and late growth stage (P<0.05). However, it showed that a promoting effect on LBR and SBR was stronger under high-concentration nitrogen treatment and in the late growth stage of Stinkweed (P<0.05). When potted for 45 days, the content of chlorophyll a in the high nitrogen group (N₃) was increased by 131.69% compared with the control group (N₀), and the increase in chlorophyll b was 787.50%. Chlorophyll a and chlorophyll b increased by 82.45% and 314.29% respectively in potted plants for 90 days. It shows that the chlorophyll synthesis ability has strong tolerance to the heterogeneous environment represented by high nitrogen levels. At the early stage of growth (45 d), the positive feedback effect on moderate-level nitrogen deposition (N=60 kg/hm²) was strong. However, with the extension of the growth period (90 d), the adaptability to high levels of nitrogen deposition (N=80 kg/hm²) was significantly improved. The result proves that the aggravation of nitrogen deposition is beneficial to its growth.