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Artocarpus heterophyllus is popular for its sweet fruit and has a very promising market, but conventional seed propagation makes it difficult to maintain its excellent traits and the grafting method has a very low survival rate. The technique of tissue culture and rapid propagation has been widely used in the propagation of many rare and valuable plants, but there are no reports of its application in the production of A. heterophyllus. In the process of tissue culture, it was found that the stem tip of A. heterophyllus was severely browned, which led to the difficulty of establishing asexual reproduction system. In order to effectively investigate the measures to overcome the browning, this experiment took the stem tips of A. heterophyllus 'Haida 2' as the explants and inoculated them into different concentrations of PVP medium after low-temperature pretreatment at different lengths for primary culture, the browning rate and germination rate were calculated after 15 d, and PPO activity, total phenol and total flavonoid content were determined to explore the inhibition of different concentrations of PVP on browning in stem the tip tissue culture of A. heterophyllus after low-temperature pretreatment. The results showed that the browning rate of A. heterophyllus explants was the lowest and the germination rate was the highest after 12-24 h low-temperature treatment of stem tip and then in 1/2MS medium supplemented with 2.0 g/L PVP. The experimental results showed that this method was beneficial to the proliferation of A. heterophyllus explants. The purpose of this experiment is to promote the establishment of technique of tissue culture and rapid propagation for A. heterophyllus, with a view to providing a theoretical basis for the construction of the A. heterophyllus seedling breeding technology system and its industrial production demonstration.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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