PDF
Full
Outline
Identification of heterozygote is a key step in the cross breeding of Lentinula edodes. A new ISSR technique (N-ISSR) was designed in this study based on discrimination between the two nucleus of the donor strain to identify quickly and accurately the authenticity of hybrid strains of L. edodes. The new ISSR was used to identify 40 hybrid strains which were from the Di-mon mating between dikaryon wild strain YX7 and spore monokaryons of cultivated strain 808, as well as microscopy observation combined with antagonistic test (MOCAT) and the traditional ISSR technique (T-ISSR) as the control. N-ISSR results showed 27 strains were confirmed to be true heterozygote as they possessed specific DNA bands from one of the nucleus of donor strain YX7, and the other 13 strains were confirmed to not be heterozygote. Among them, 11 strains were strain YX7 itself as they possessed both specific DNA bands from the two nucleus of strain YX7, and 2 strains were spore monocaryons of strain 808 as they had no any specific DNA bands of donor strain YX7. Compared with MOCAT, N-ISSR could thorough identify all the 27 heterozygote strains, which included the 19 strains identified as heterozygote, the 6 strains unable to be identified and 2 strains incorrectly identified as non-heterozygote by MOCAT. N-ISSR could identify all the 27 heterozyote stains and 13 non-heterozyote stains with simple primer, and could classify the heterozygote strains into two groups according to the type of nucleus received from the donor. On contrast, T-ISSR needed 4 primers to identify all the 27 heterozygote strains, and was unable to con firm the other 13 stains as non-heterozygote stains, and was unable to classify the heterozygote strains into two groups. In conclusion, N-ISSR was an effective tool to be used for fast, extensive and accurate identification of heterozygote and non-heterozygote strains of L. edodes, and would be a technical support for further study on the cross breeding and genetic analysis of L. edodes.
PDF
37
9
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
