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Hirschmanniella spp. are migratory endoparasitic nematodes which are parasitic in the root of rice. They are widely distributed in different types of fields and have become serious parasitic nematodes to rice. The plant-parasitic nematode Hirschmanniella mucronate is one of the most serious rice nematodes in Jiangxi, China. At present, breeding and planting other disease-resistant varieties is the most economical and effective measure to control the disease. The significant up-regulated OsECH1 gene was screened by transcriptome analysis of healthy susceptible rice 'Bawangbian' root tissues and susceptible rice 'Bawangbian' root tissues infected by H. mucronata. Phylogenetic analysis of OsECH1 and its homologous proteins showed that OsECH1 and other homologous proteins had the same structure, including the same conserved domain PLN02874. And OsECH1 belonged to the ECH protein family. Besides, five homologous genes were found in the genome of 'Nipponbare' group and OsECH1 was located in Chr1. The full length of OsECH1 was cloned and the recombinant expression vector was constructed by homologous recombination technique. Meanwhile, the transgenic rice was transformed by GV1301. RT-qPCR was used to analyze the effect of OsECH1 gene expression level on the infection of H. mucronate in rice. The results showed that overexpressing OsECH1 in rice could reduce the infection of H. mucronata, while silencing OsECH1 in rice could significantly increase the infection of H. mucronata. The results of subcellular localization of OsECH1 showed that the protein was expressed in nucleus. And the results of His-tag pull down demonstrated that the protein may interacted with five defence-related proteins. The five defense-related proteins belonged to vATP-synt_E protein family, Ricin-B protein family, catalase and B_lectin protein family respectively. Meanwhile, RT-qPCR results showed that OsECH1 had regulatory effects on the five proteins. The results would provide a basis for further understanding the function of OsECH1 gene, and for understanding the molecular mechanism of OsECH1.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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