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This paper aimed to clarify the effect of different microbial agents on pepper blight and the influence of rhizosphere soil bacterial community structure, and to provide a green prevention technical basis for reducing the occurrence of pepper blight. Six microbial agents, including Bacillus subtilis, Trichoderma harzianum, Pseudomonas fluorescens and Streptomyces enissocaesllis were selected as the main research objects to study the effects on the control of pepper blight and the bacterial community structure in rhizosphere soil under pot experiment by high-throughput sequencing. A1 (1×108 CFU/g of B. subtilis microcapsule) and A2 (1×108 CFU/g of T. harzianum water-dispersed granule) had the best control effect, and the control effect was 85.58% and 81.97%, respectively, and others the control effects of A3 (5×108 CFU/g Pseudomonas fluorescence granules). The best control effect of A4 (4×109 CFU/g S. enissocaesllis NBF715 powder), A5 (1×1010 CFU/g B. subtilis wettable powder), A6 (3×108 CFU/g T. harzianum wettable powder) and A7 (50% carbendazim wettable powder) was 47.17%, 49.12%, 30.60%, 39.86%, 25.83%, respectively. Based on the high-throughput sequencing analysis, the bacterial community composition of pepper rhizosphere soil at phylum level, different microbial agents increased the proportion of Proteobacteria and Bacteroidota, especially A1 and A2 significantly increased the richness of Proteobacteria and Bacteroidetes. At the family level, the richness of Chitinophagaceae, Steroidobacteriaceae and Micrococcaceaee were improved by microbial agent treatment, and significant difference was found between A1 and A2 treatments. The total number of bacterial OTUs and the number of unique bacterial OTUs in the rhizosphere soil of Capsicum treated with A1, A2, A7, A8 (treatment of inoculated P. capsici) and CK (water) were analyzed by Venn diagram. The total number of bacterial OTUs and the number of unique dominant bacteria in the rhizosphere soil of capsicum increased in the A1 and A2 treatments, while the richness and diversity of soil bacteria decreased in the A7 treatment. In conclusion, the control effect of A1 and A2 on pepper blight was more than 80%, and the richness and diversity of unique dominant bacteria in the rhizosphere soil of pepper increased, and the ability of pepper to resist P. capsici infection was enhanced. A1 and A2 could be used as agents to control pepper Phytophthora blight. At the same time, the use of carbendazim reduced the richness and diversity of soil bacteria, and the pathogen could invade the root of pepper more quickly after the reduction of microbial diversity.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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