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Phenotypic Characteristics and Genetic Analysis of bgl-2, a Bright Green Leaf Mutant Oryza sativa L.
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Qiuyun LIN, Zhenyu XIE, Wei HU, Yujie ZHOU, Kaiyi LONG, Zhizhou HE*
Chinese Journal of Tropical Crops | 2023, 44(6) : 1091 - 1096
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Chinese Journal of Tropical Crops | 2023, 44(6): 1091-1096
Omics & Biotechnology
Phenotypic Characteristics and Genetic Analysis of bgl-2, a Bright Green Leaf Mutant Oryza sativa L.
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Qiuyun LIN, Zhenyu XIE, Wei HU, Yujie ZHOU, Kaiyi LONG, Zhizhou HE*
Affiliations
  • Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
Published: 2023-06-25 doi: 10.3969/j.issn.1000-2561.2023.06.002
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Leaf color mutants are not only an ideal material for the study of rice functional genomics, but also of great significance for hybrid rice breeding. They can also be used as ornamental rice, committed to building rural leisure agricultural sightseeing spots and promoting rural revitalization. Based on a rice bright green leaf mutant bgl-2 obtained by radiation mutagenesis in the early stage of our research group, this study compared the difference in leaf color between the mutant bgl-2 and the wild-type SIPI, and showed that the mutant bgl-2 showed a bright green color different from the wild-type SIPI. The content of photosynthetic pigments in leaves was determined by 95% ethanol extraction at tillering stage. The results showed that the chlorophyll content of mutant bgl-2 was not significantly different from that of wild-type SIPI. By observing the other plant morphology of mutant bgl-2, it was found that the leaf tips of its flag leaf and the last two leaves were bent, and the flag leaf was significantly shorter than that of wild-type SIPI, and its plant height was also significantly shorter than that of wild-type SIPI. It was found that the 1000 grain weight, grain length and grain width of mutant bgl-2 was significantly smaller than those of wild-type SIPI, but its length width ratio was not significantly different from that of wild-type SIPI. The structure of flag leaf cells of wild-type SIPI and mutant bgl-2 at tillering stage was observed by the transmission electron microscope. The results showed that the cell wall of mutant bgl-2 was significantly thickened, but the thickness of outer membrane of cell membrane was significantly reduced, the inner membrane was almost degraded, and the lamellar structure of thylakoid in chloroplast ultrastructure was looser than that of wild-type SIPI. F1 plants were obtained by hybridization with mutant bgl-2 as female parent and wild-type SIPI as male parent. The investigation found that its leaves were normal green, and the morphology of flag leaf and inverted two leaves were normal, while the F2 population after self pollination isolated normal green leaf seedlings and bright green leaf seedlings with obvious leaf color differences. The segregation ratio of normal leaf color plants and bright green leaf plants in the population was counted, which showed that it conformed to the single gene model of 3 : 1. The above results clarify the phenotype and genetic characteristics of the mutant bgl-2, and provide a theoretical basis for the study of the molecular regulation mechanism of the mutant bgl-2 and promoting its application in rice breeding. The location cloning and functional analysis of BLG-2 gene will be carried out later.

rice  /  bright green leaf  /  mutant  /  bgl-2  /  phenotype  /  inheritance
Qiuyun LIN, Zhenyu XIE, Wei HU, Yujie ZHOU, Kaiyi LONG, Zhizhou HE. Phenotypic Characteristics and Genetic Analysis of bgl-2, a Bright Green Leaf Mutant Oryza sativa L.[J]. Chinese Journal of Tropical Crops, 2023 , 44 (6) : 1091 -1096 . DOI: 10.3969/j.issn.1000-2561.2023.06.002
Year 2023 volume 44 Issue 6
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doi: 10.3969/j.issn.1000-2561.2023.06.002
  • Receive Date:2022-07-04
  • Online Date:2026-03-05
  • Published:2023-06-25
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  • Received:2022-07-04
  • Revised:2022-08-07
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    Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
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表12种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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