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Cymbidium eburneum and Cymbidium insigne are species endemic to Hainan exclusively and belong to the endangered species. 23 F1 hybrid progenies from C. eburneum and C. insigne were used to accurately verify hybrids, to clarify the genetic relationships between hybrid progenies and the parents with SSR molecular markers. Eight pairs of primers were screened out from 48 pairs of primers using PCR, and 23 hybrid progenies were confirmed to be true hybrids, with the true rate of hybrid progenies100%. 23 hybrids populations identified by primers Cym45, Hub131 and Hub8 had both parental specific loci. 23 hybrid populations identified by primers Hub125 had only father specific loci, Cym9 had a new specific loci. Primer Cym25, Hub125 and Cym47 were low polymorphi [polymorphism information content (PIC)<0.25], primer Cym45, Hub131, Hub8 and Cym9 were middle-highly polymorphi (0.25<PIC<0.5). The PIC of primer Cym172 was 0.59, indicating that it was highly polymorphi, and could be used as the preferred primer for fingerprint construction of the progenies of C. eburneum × C. insigne. UPGMA cluster analysis results showed that when the genetic coefficient was 0.8111, the studied germplasms could be divided into three categories. Among which the parents constituted two categories, and the hybrid population belonged to the third. The hybrid population could be divided into three clusters, X7 was a clade, X6 and X21 were clustered together, and the other 20 F1 hybrid population s were the third. This study showed that SSR molecular markers could be used in hybrid identification and genetic diversity analysis of orchids species endemic to Hainan, which could provide an effective foundation and data base for orchid breeding and new species selection in Hainan. At the same time, the PIC of SSR molecular markers was an important indicator reflecting the ability of molecular markers. The middle-highly polymorphic primers and highly polymorphic primers developed in this study could lay a foundation for the construction of Hainan wild orchid gene atlas and the establishment and development of Hainan wild orchid germplasm bank.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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