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Application of Cassava MNP Markers in Variety Identification
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Renjing WAN1, 2, Qiong LI3, Xincheng ZHOU4, Lun LI1, 2, Tiantian LI1, 2, Junfei ZHOU1, 2, Sha LI5, 6, Hai PENG1, 2, Weixiong ZHANG2, 7, Zhiwei FANG1, 2, *
Chinese Journal of Tropical Crops | 2023, 44(12) : 2417 - 2423
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Chinese Journal of Tropical Crops | 2023, 44(12): 2417-2423
• Special Focus: Resources and Utilization of Cassava •
Application of Cassava MNP Markers in Variety Identification
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Renjing WAN1, 2, Qiong LI3, Xincheng ZHOU4, Lun LI1, 2, Tiantian LI1, 2, Junfei ZHOU1, 2, Sha LI5, 6, Hai PENG1, 2, Weixiong ZHANG2, 7, Zhiwei FANG1, 2, *
Affiliations
  • 1.School of Life Science, Jianghan University, Wuhan, Hubei 430056, China
  • 2.Institute for Systems Biology, Jianghan University, Wuhan, Hubei 430056, China
  • 3.Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
  • 4.Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
  • 5.Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei 443100, China
  • 6.Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei 443100, China
  • 7.Faculty of Health and Social Sciences, The Hong Kong Polytechnic University, Hong Kong 100872, China
Published: 2023-12-25 doi: 10.3969/j.issn.1000-2561.2023.12.007
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MNP (multiple nucleotide polymorphism, MNP) is a new molecular marker technology emerged with the development of molecular marker technology. While genotyping by target sequencing (GBTS) amplifies only one SNP site in one amplicon, MNP can amplify multiple SNP sites simultaneously in one amplicon based on GBTS. This technology has the features of low cost, high detection efficiency, flexible application and wide adaptability, Compared with SNP, MNP has better ability to differentiate varieties. In order to establish a rapid, accurate and efficient method for cassava variety identification and to screen MNP molecular markers suitable for cassava variety identification, this study screened highly polymorphic regions and designed primers in the whole genome range, and finally obtained 623 cassava MNP marker loci in the whole genome range, the cassava MNP markers were evaluated using 28 cassava varieties. The results showed that the MNP marker typing reproducibility was up to 100%. 4.07±1.68 alleles were detected in 28 cassava varieties, with a maximum of 12 alleles. When all cassava varieties were compared one by a one, 99.47%(376/378) of the differences between pairs were greater than 46%, with the proportion ranging from 0.3% to 81.0%, with a mean value of 71.78%. Meanwhile, MNP markers can be used for identification in the breeding process. In conclusion, the cassava MNP molecular markers developed in this study have high reproducibility, polymorphism and variety differentiation ability, and could be widely used for research on germplasm diversity, new variety breeding and variety identification of cassava.

cassava  /  molecular marker  /  MNP  /  variety identification
Renjing WAN, Qiong LI, Xincheng ZHOU, Lun LI, Tiantian LI, Junfei ZHOU, Sha LI, Hai PENG, Weixiong ZHANG, Zhiwei FANG. Application of Cassava MNP Markers in Variety Identification[J]. Chinese Journal of Tropical Crops, 2023 , 44 (12) : 2417 -2423 . DOI: 10.3969/j.issn.1000-2561.2023.12.007
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Article Info
doi: 10.3969/j.issn.1000-2561.2023.12.007
  • Receive Date：2022-11-02
  • Online Date：2026-03-05
  • Published：2023-12-25
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History
  • Received：2022-11-02
  • Revised：2022-12-22
Funding
Affiliations
    1.School of Life Science, Jianghan University, Wuhan, Hubei 430056, China
    2.Institute for Systems Biology, Jianghan University, Wuhan, Hubei 430056, China
    3.Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
    4.Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
    5.Hubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei 443100, China
    6.Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei 443100, China
    7.Faculty of Health and Social Sciences, The Hong Kong Polytechnic University, Hong Kong 100872, China
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Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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