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Characteristics and Expression Analysis Of Chalcone Synthase MeCHS Family in Cassava
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Feifei AN1, 2, Jianxiong QI1, Songbi CHEN2, Xiuqin LUO1, Jie CAI2, Jingjing XUE2, *
Chinese Journal of Tropical Crops | 2023, 44(12) : 2384 - 2391
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Chinese Journal of Tropical Crops | 2023, 44(12): 2384-2391
• Special Focus: Resources and Utilization of Cassava •
Characteristics and Expression Analysis Of Chalcone Synthase MeCHS Family in Cassava
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Feifei AN1, 2, Jianxiong QI1, Songbi CHEN2, Xiuqin LUO1, Jie CAI2, Jingjing XUE2, *
Affiliations
  • 1.Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Conservation and Utilization of Cassava Germplasm Resources, Ministry of Agriculture and Rural Affairs, Haikou, Hainan 571101, China
  • 2.Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya, Hainan 572025, China
Published: 2023-12-25 doi: 10.3969/j.issn.1000-2561.2023.12.003
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Chalcone synthase (CHS) is the first limiting enzyme in flavonoid synthesis pathway and plays an important role in flower color formation, growth and development, and abiotic stress. In order to clarify the characteristics of cassava MeCHS gene family and its expression in cassava postharvest physiological deterioration (PPD), the MeCHS gene family was analyzed using bioinformatics, including physicochemical property and subcellular location. qRT-PCR was carried out to detect the expression levels of MeCHS genes in different varieties, different tissues and PPD process. The genes with significant relative expression were selected for cloning and subcellular localization verification. A total of 5 MeCHS gene members were identified in the cassava genome. The secondary structure analysis showed that the MeCHS gene family was mainly composed of α-helices and random coils. Tissue specific expression indicated that the expression levels of MeCHS genes were higher in stems. The major expressed MeCHS genes in cassava were MeCHS1, MeCHS2 and MeCHS3; and the expression levels of these three genes were gradually increase with PPD. MeCHS1, MeCHS2 and MeCHS3 were successfully cloned; and fluorescence transient expression showed that MeCHS1, MeCHS2 and MeCHS3 were localized in the cytoplasm, which was consistent with the predicted results. This study will provide a theoretical basis for further revealing the function of MeCHS gene family and improving the PPD tolerance of cassava.

cassava  /  chalcone synthase  /  qRT-PCR  /  cloning  /  expression analysis
Feifei AN, Jianxiong QI, Songbi CHEN, Xiuqin LUO, Jie CAI, Jingjing XUE. Characteristics and Expression Analysis Of Chalcone Synthase MeCHS Family in Cassava[J]. Chinese Journal of Tropical Crops, 2023 , 44 (12) : 2384 -2391 . DOI: 10.3969/j.issn.1000-2561.2023.12.003
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Year 2023 volume 44 Issue 12
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Article Info
doi: 10.3969/j.issn.1000-2561.2023.12.003
  • Receive Date：2023-07-12
  • Online Date：2026-03-05
  • Published：2023-12-25
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History
  • Received：2023-07-12
  • Revised：2023-07-17
Funding
Affiliations
    1.Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Conservation and Utilization of Cassava Germplasm Resources, Ministry of Agriculture and Rural Affairs, Haikou, Hainan 571101, China
    2.Sanya Research Institute, Chinese Academy of Tropical Agricultural Sciences, Sanya, Hainan 572025, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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