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Noni fruit is a characteristic fruit of Hainan with a variety of medicinal effects, but there are few studies on its plant proteins. In the present study, two-dimensional gel electrophoresis (2-DE gel) technology based on the BPP+phenol extraction method was used to extract and separate the proteins of noni fruits at different ripening stages including bud stage, half ripe stage, and mature stage by optimizing the experimental conditions to obtain the fingerprint of plant proteins. The gel images were analyzed by Image Master 5.0 software and some proteins with high expression were screened out. The enzymatic hydrolysates of the proteins were further identified by using MALDI-TOF-MS technology. The functional analysis of the highly expressed proteins of three kinds of noni fruits were preliminarily carried out by bioinformatics methods. The results indicated that there were respectively forty four, forty nine, and forty highly expressed proteins at three maturites noni fruits. Among the proteins, there were respectively thirty three, twenty two and thirty nine proteins by mass spectrum identification, which subordinated to various types of protein with different plant functions. Moreover, significant difference existed in the highly expressed proteins of different maturity stages, which involved in a variety of proteins such as chloroplastic ligase, cysteine proteinase, protein kinase, ubiquitin ligase, and auxin response factors. It could provided a scientific and reasonable theoretical guidance for the further development and utilization of the plant protein of noni fruits.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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