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Phosphorus is an indispensable element in the process of plant growth and development, but it is mostly in the form of ineffective bound phosphorus in the field. The microorganisms in soil can convert the ineffective phosphorus into free effective phosphorus, and fully improve the utilization rate of phosphorus. In this study, a phosphate-solubilizing fungus XZY3PSF was isolated and purified from the corn rhizosphere soil using the phosphorus-solubilized halo method, and the strain was identified. The activity of the strain to dissolve 2.5-20.0 g/L tricalcium phosphate and the phosphate solubilization ability of 10-105 spores/mL to tricalcium phosphate in 1-25 d were analyzed. The mineralization ability of 2.5 g/L and 5.0 g/L calcium phytate was evaluated, and the interaction between it and Bacillus amyloliquefaciens and the pathogen of banana Fusarium wilt disease was evaluated. Based on the morphological characteristics combined with ITS and β-tubulin gene sequences, the strain XZY3PSF was identified as Talaromyces purpureogenus. The highest content of available phosphorus dissolved by phosphate-solubilizing strain under different concentrations of tricalcium phosphate was between 500 and 570 mg/L. The content of available phosphorus in the medium decreased with the prolongation of culture time. The decreasing range of the available phosphorus content in the tricalcium phosphate medium was weakened. On the 25th day, the available phosphorus content in the 15.0 and 20.0 g/L tricalcium phosphate medium was significantly higher than that of the other concentrations. With the increase of the spore concentration in the medium, higher peak value of the available phosphorus content in the culture medium appeared, and its decreasing range expanded with the increase of the spore concentration. During the test period, with the extension of culture time, the mineralization ability of XZY3PSF to calcium phytate was stronger. XZY3PSF and Bacillus X5 had antagonistic effects on the pathogen of banan Fusarium wilt, but the phosphate solubilizing fungus and Bacillus spp. also antagonized each other. The research results would lay a foundation for the development of microbial fertilizers composed of phosphorus-solubilizing fungi and antagonistic bacteria. At the same time, the mineralization of XZY3PSF on calcium phytate would provide a basic theoretical basis for subsequent studies on whether it can degrade organophosphorus pesticides.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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