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During the evolution from lower plants to higher plants, lignification is the key to adapt to the terrestrial environment, while lignin deposition in the cell wall is an important manifestation of lignification, and MYB61 plays an important role in lignin synthesis pathway. In order to explore the molecular mechanism of MYB61 in lignin synthesis of Impatiens chlorosepala and Impatiens uliginosa, MYB61 genes, named IcMYB61 and IuMYB61 in this study, were cloned by using the RT-PCR method. The full-length cDNA was 1035 bp and 1002 bp, encoding 344 aa and 333 aa, containing an intron of 84 bp and 66 bp, respectively. Physicochemical analysis suggested that IcMYB61 and IuMYB61 were unstable hydrophilic protein. Domain analysis of protein showed that IcMYB61 and IuMYB61 contained two typical SANT conserved domains. The comparison of amino acid sequences of MYB61 with that of other species showed that there were highly conserved R2 and R3 regions at the N-terminal, which was speculated to be R2R3-MYB gene. Based on the phylogenetic analysis, it found that IcMYB61 and IuMYB61 were clustered together with I. glandulifera, confirming that the same genus is more closely related. The qPCR showed that IcMYB61 and IuMYB61 were expressed in three different parts of the two periods, and the expression levels were the lowest in roots. In I. chlorosepala, the highest expression of IcMYB61 was in the leaves at the seedling stage, and the highest expression level was in the stem at the mature stage. In I. uliginosa, the expression of IuMYB61 was the highest in the stems of both periods. By measuring the total lignin content in stems and leaves of the two species, it was found that the lignin content of both species was the highest in stems, which was consistent with the expression trend of MYB61. It is speculated that MYB61 mainly regulates the biosynthesis of lignin in the stems of I. chlorosepala and I. uliginosa. The above-mentioned results would provide some basic data and scientific basis for exploring the regulation mechanism of MYB61 on the lignin synthesis of Impatiens and the culture of new varieties.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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