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Three-line matching is the main research direction of pepper breeding, identification and development of restorer gene linkage markers in pepper is a difficult problem in restorer line breeding. In this study, the F2 segregation population was constructed by using 014A and 014C as parents, the segregation ratio of fertility and sterility was 3∶1 by χ2 test, indicating that CMS fertility recovery trait of capsicum was controlled by one pair of dominant genes. Using the BSA method to construct the mixed pool of fertile and sterile DNA, the whole genome was re-sequenced and compared with the reference genome, by SNP-index Method and Fisher Test, the cytoplasmic male sterility restorer gene was mapped in the region of 1.44-8.28 Mb at the tip of the chromosome 6. The primers were designed according to the SNP/InDel difference between parents, and the polymorphic primers were screened in both parents and extreme pools, and the molecular markers PP5 and OP59 were obtained. The accuracy of the two markers was 100% in extreme population validation; The marker OP59 was a co-dominant marker, which was located at the candidate gene T459-15819 interval of 17 232 bp. The accuracy of the marker was 100% in the sterile population and 97.21% in the fertile population; PP5 was located 318 bp downstream of the candidate gene T459-15819 of the restorer gene. The accuracy of PP5 was 100% in both sterile and homozygous fertile populations. In this study, we obtained two markers closely linked with the restorer gene, which would lay the foundation for accelerating the selection of CMS restorer lines in pepper.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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