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Pathogenicity and Infection Behavior of Heterorhabditis bacteriophora H06 Strain to Different States of Rhynchophorus ferrugineus Oliver
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Chaojun LYU, Jianchao ZHAO, Baozhu ZHONG*, Chuyu ZHANG, Tuo CHEN, Wenlian LI
Chinese Journal of Tropical Crops | 2023, 44(8) : 1698 - 1703
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Chinese Journal of Tropical Crops | 2023, 44(8): 1698-1703
Plant Protection & Bio-safety
Pathogenicity and Infection Behavior of Heterorhabditis bacteriophora H06 Strain to Different States of Rhynchophorus ferrugineus Oliver
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Chaojun LYU, Jianchao ZHAO, Baozhu ZHONG*, Chuyu ZHANG, Tuo CHEN, Wenlian LI
Affiliations
  • Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wenchang, Hainan 571339, China
Published: 2023-08-25 doi: 10.3969/j.issn.1000-2561.2023.08.020
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In order to clearify the potential of entomopathogenic nemotodes (EPNs) on the control of Rhynchophorus ferrugineus, the pathogenucity of Heterorhabditis bacteriophora H06 against all stages of R. ferrugineus and the behavior of the invading red palm weevil larvae was studied using laboratory bioassay methods. The results showed that the mortality of eggs was the highest in all stages after R. ferrugineus was infected by EPNs, and LC50 was 104.85 IJs/mL, followed by 1-2 instar larvae, 3-4 instar larvae and 5-6 instar larvae, LC50 was 165.34 IJs/mL, 215.31 IJs/mL and 292.26 IJs/mL respectively; No significant differences were observed in the sensitivity between 7-10 instar larvae and pupae to EPNs. Among the effects of inoculation site on the mortality of different instar larvae, the mortality of the 3rd instar larvae was the highest when inoculated with spiracle and anus, which was 91.11% and 78.89% respectively, while the lethal rate of inoculation with mouthparts was the highest in the treatment of the 6th and 8th instar larvae. No significant difference presented in the mortality of different instar R. ferrugineus larvae when treated with vent inoculation. The R. ferrugineus body color changed to red or reddish brown, no spontaneous response when touching, and the body wall of R. ferrugineus shrinked after treated by EPNs. When the nematode approached the spiracle of R. ferrugineus larval, it stayed around the spiracle for a moment, and finally entered the insect body, and the number of EPNs successfully invaded was about 33.33%.

Heterorhabditis bacteriophora  /  pathogenicity  /  Rhynchophorus ferrugineus Oliver  /  morphological
Chaojun LYU, Jianchao ZHAO, Baozhu ZHONG, Chuyu ZHANG, Tuo CHEN, Wenlian LI. Pathogenicity and Infection Behavior of Heterorhabditis bacteriophora H06 Strain to Different States of Rhynchophorus ferrugineus Oliver[J]. Chinese Journal of Tropical Crops, 2023 , 44 (8) : 1698 -1703 . DOI: 10.3969/j.issn.1000-2561.2023.08.020
Year 2023 volume 44 Issue 8
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doi: 10.3969/j.issn.1000-2561.2023.08.020
  • Receive Date:2022-08-01
  • Online Date:2026-03-05
  • Published:2023-08-25
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  • Received:2022-08-01
  • Revised:2022-09-15
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    Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wenchang, Hainan 571339, China
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https://castjournals.cast.org.cn/joweb/rdzwxb/EN/10.3969/j.issn.1000-2561.2023.08.020
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表12种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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