In order to assess the breeding value of HoCP series sugarcane germplasms introduced from USA, 12 HoCP sugarcane clones (HoCP00-1142, HoCP01-517, HoCP01-564, HoCP02-610, HoCP02-623, HoCP03-704, HoCP03-708, HoCP03-716, HoCP05-902, HoCP07-613, HoCP07-617 and HoCP92-648) were used as core parents in 73 sugarcane cross combinations. The effect on combining ability, economic breeding values of height, diameter, effective stem number, sugar content and cane yield for the 11 HoCP sugarcane parents and 73 F₁ population were estimated using the family test method. Significant differences were found for height among HoCP males and cross combinations, whereas no significant differences were detected for height among HoCP females, suggesting that the plant height was affected by only male and cross combination. For the stem diameter, effective stem number, sugar content and cane yield, there were significant differences existed among different HoCP female, male and cross combination, indicating that the four characters were affected by female, male and cross combination. Among the 12 clones, the highest general combining ability for height, diameter, effective stem number, sugar content and cane yield appeared in HoCP03-704, HoCP02-623, HoCP02-610, HoCP07-617, and HoCP92-648 as female, respectively, while HoCP03-704, HoCP02-623, HoCP07-617, HoCP03-708 and HoCP02-623 as male possessed the highest general combining ability for height, diameter, effective stem number, sugar content and cane yield, respectively. The better economy breeding value (EBV) and general combining ability (GCA) in cane yield and sugar content were detected in HoCP02-623, HoCP03-708 and HoCP92-648 as the female, and HoCP01-517and HoCP07-617 as the male, which indicated that the clones could be used as excellent female and male with high-yield and hight-sugar, respectively. Among the 73 cross combinations, the highest special combining ability for height, diameter, effective stem number, sugar content and cane yield appeared in HoCP03-704×'Neijiang 03-218', 'Yuetang 00-236'×HoCP00-1142, 'Yacheng 07-71'×HoCP05-902, 'Yuetang 00-236'×HoCP03-708, HoCP01-564×'Guitang 92-66', respectively. A total of 38 cross combinations showed possitive EBV. The combinations 'Yuetang 94-128'×HoCP01-517, HoCP01-564×'Guitang 92-66', 'Guitang 02-467'×HoCP01-564 had better special combining ability in cane yield, sugar content and EBV than the others, which could be considered as the most important productive families in future breeding program. However, HoCP03-716×'Funong 02-6404', 'Yuetang 99-66'×HoCP01-564 and 'Yuenong 73-204'×HoCP01-564 showed lower special combining ability in five characters and lower EBV than other combinations, suggesting that the combinations did not suit for using as productive families in future breeding program. Therefore, the results would provide helpful information for using HoCP sugarcane parents in cross combination.