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Industrial Tissue Culture Speed Propagation of Curcuma alismatifolia 'Majeo Impress Red'
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Jianjun TAN1, Yuanjun YE1, Jinmei LIU1, Genfa ZHU1, Weibing XU2, Xiaodong CUI2, *, Yechun XU1, *
Chinese Journal of Tropical Crops | 2023, 44(2) : 347 - 357
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Chinese Journal of Tropical Crops | 2023, 44(2): 347-357
• Plant Cultivation, Physiology & Biochemistry •
Industrial Tissue Culture Speed Propagation of Curcuma alismatifolia 'Majeo Impress Red'
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Jianjun TAN1, Yuanjun YE1, Jinmei LIU1, Genfa ZHU1, Weibing XU2, Xiaodong CUI2, *, Yechun XU1, *
Affiliations
  • 1.Environmental Horticulture Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Key Lab of Ornamental Plant Germplasm Innovation and Utilization, Guangzhou, Guangdong 510640, China
  • 2.Guangdong Dongjiang Forest Farm, Heyuan, Guangdong 517465, China
Published: 2023-02-25 doi: 10.3969/j.issn.1000-2561.2023.02.014
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In order to improve the technological process of industrial tissue culture speed propagation of Curcuma alismatifolia 'Majeo Impress Red', and provide a reference for the large-scale production, we used different organs as the explants to study the bud multiplication, proliferation, rooting culture and transplanting. Six different explants including corm, storage root, corm shoot, lateral bud, flower bud and tender leaves were employed in this study. Based on the MS medium with different concentration of 6-BA and NAA, the process of tissue culture speed propagation was optimized. The best explants were corm shoot and lateral bud. The best medium for induction culture was MS + 6-BA 3 mg/L +NAA 0.1 mg/L + sucrose 30 g/L + carrageenans 7 g/L. The best medium for proliferation was MS + 6-BA 3 mg/L +NAA 0.1 mg/L + sucrose 30 g/L + carrageenans 7 g/L. The best medium for rooting was 1/2 MS + NAA 0.1 mg/L +sucrose 20 g/L + carrageenans 7 g/L. When the seedlings were pluged in tray and transplanted in flowerpot, the optimum medium was tiny peat∶tiny coco coir = 1∶1 and the survival rate was over 95%. Eight EST-SSR markers were employed to amplified the maternal parent and 20 randomly selected tissue culture seedlings, which supported the obsence of DNA-level variations. We screened the most appropriate explants and culture medium for C. alismatifolia 'Majeo Impress Red', optimized the technological process and set up a technical system of industrial tissue culture speed propagation.

Curcuma alismatifolia  /  explants  /  clustered shoots induction  /  tissue culture  /  large-scale production
Jianjun TAN, Yuanjun YE, Jinmei LIU, Genfa ZHU, Weibing XU, Xiaodong CUI, Yechun XU. Industrial Tissue Culture Speed Propagation of Curcuma alismatifolia 'Majeo Impress Red'[J]. Chinese Journal of Tropical Crops, 2023 , 44 (2) : 347 -357 . DOI: 10.3969/j.issn.1000-2561.2023.02.014
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Year 2023 volume 44 Issue 2
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Article Info
doi: 10.3969/j.issn.1000-2561.2023.02.014
  • Receive Date：2022-02-10
  • Online Date：2026-03-05
  • Published：2023-02-25
Article Data
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History
  • Received：2022-02-10
  • Revised：2022-06-08
Funding
Affiliations
    1.Environmental Horticulture Research Institute, Guangdong Academy of Agricultural Sciences/Guangdong Key Lab of Ornamental Plant Germplasm Innovation and Utilization, Guangzhou, Guangdong 510640, China
    2.Guangdong Dongjiang Forest Farm, Heyuan, Guangdong 517465, China
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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