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Effect of Methyl Jasmonate on Laticifer Cell Differentiation of Hevea brasiliensis Anther Calli
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Zihan GUO1, 2, Deguan TAN2, *, Dongyi HUANG1, Lili FU2, Xuepiao SUN2, Jiaming ZHANG2, *
Chinese Journal of Tropical Crops | 2023, 44(2) : 310 - 316
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Chinese Journal of Tropical Crops | 2023, 44(2): 310-316
• Plant Cultivation, Physiology & Biochemistry •
Effect of Methyl Jasmonate on Laticifer Cell Differentiation of Hevea brasiliensis Anther Calli
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Zihan GUO1, 2, Deguan TAN2, *, Dongyi HUANG1, Lili FU2, Xuepiao SUN2, Jiaming ZHANG2, *
Affiliations
  • 1.College of Tropical Crops, Hainan University, Haikou, Hannan 570228, China
  • 2.Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences/Hainan Academy of Tropical Agricultural Resources, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Tropical Crop Biology and Genetic Resources, Ministry of Agriculture and Rural Affairs, Haikou, Hainan 571101, China
Published: 2023-02-25 doi: 10.3969/j.issn.1000-2561.2023.02.010
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Laticifer cells present in aging calli of Hevea brasiliensis. Methyl jasmonate (MeJA) and jasmonic acid (JA) belong to jasmonates (JAs). However, the effect of MeJA on laticifer cell differentiation of the rubber tree anther calli is unclear. In the present paper, the effect of MeJA on laticifer cell differentiation of anther calli was systematically studied. MeJA added to media in the range of 0-1 mg/L did not influence the growth of anther calli, while higher concentration of MeJA in media in the range of 2-3 mg/L severely inhibited callus growth. MeJA concentration in media was negatively correlated with callus growth. Histochemical sections showed that low concentration of MeJA in media greatly promoted the differentiation of callus laticifer cells and remarkably increased the occurring frequency, while high concentration of MeJA in media gravely impeded callus laticifer cell differentiation and strikingly decreased the occurring frequency. Among them, 1 mg/L MeJA in media was the optimal concentration to improve the differentiation of laticifer cells in calli. qPCR results showed that when the media were supplemented with MeJA, the change trend of the expression level of genes that was essential in latex biosynthesis, such as small rubber particle protein (SRPP), rubber elongation factor (REF), and cis-prenyl transferase (CPT), was basically consistent with that of the occurring frequency of callus laticifer cells, which further confirmed that MeJA promoted the differentiation of callus laticifer cells. Transmission electron microscopy observation showed that callus laticifer cells contained some specific organelles, such as rubber particles and lutoids. The results of this paper are of great significance for promoting the application of rubber tree callus in the study of laticifer differentiation, and for developing rubber tree novel yield-increasing reagents.

Hevea brasiliensis Muell. Arg.  /  callus  /  laticifer cell differentiation  /  methyl jasmonate  /  gene expression
Zihan GUO, Deguan TAN, Dongyi HUANG, Lili FU, Xuepiao SUN, Jiaming ZHANG. Effect of Methyl Jasmonate on Laticifer Cell Differentiation of Hevea brasiliensis Anther Calli[J]. Chinese Journal of Tropical Crops, 2023 , 44 (2) : 310 -316 . DOI: 10.3969/j.issn.1000-2561.2023.02.010
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Year 2023 volume 44 Issue 2
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Article Info
doi: 10.3969/j.issn.1000-2561.2023.02.010
  • Receive Date：2022-03-01
  • Online Date：2026-03-05
  • Published：2023-02-25
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History
  • Received：2022-03-01
  • Revised：2022-05-08
Funding
Affiliations
    1.College of Tropical Crops, Hainan University, Haikou, Hannan 570228, China
    2.Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences/Hainan Academy of Tropical Agricultural Resources, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Tropical Crop Biology and Genetic Resources, Ministry of Agriculture and Rural Affairs, Haikou, Hainan 571101, China
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https://castjournals.cast.org.cn/joweb/rdzwxb/EN/10.3969/j.issn.1000-2561.2023.02.010
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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