Home Journals Articles Updates About
CN
image
收藏切换
Construction of Yeast Two-hybrid Normalized Library and Screening of Interaction Proteins of PnRXLR5863 in Agave hybrid No.11648
收藏切换
PDF
Ziping YANG, Qian YANG, Xun WANG, Zhi KE, Zhiwei LU, Yanmei ZHANG, Huibang SHEN, Wenzhao ZHOU*
Chinese Journal of Tropical Crops | 2023, 44(2) : 246 - 253
Less
收藏切换
Chinese Journal of Tropical Crops | 2023, 44(2): 246-253
• Omics & Biotechnology •
Construction of Yeast Two-hybrid Normalized Library and Screening of Interaction Proteins of PnRXLR5863 in Agave hybrid No.11648
Full
Ziping YANG, Qian YANG, Xun WANG, Zhi KE, Zhiwei LU, Yanmei ZHANG, Huibang SHEN, Wenzhao ZHOU*
Affiliations
  • South Subtropical Crops Institute, Chinese Academy of Tropical Agricultural Sciences/Zhanjiang Key Laboratory of Tropical Crop Genetic Improvement, Zhanjiang, Guangdong 524091, China
Published: 2023-02-25 doi: 10.3969/j.issn.1000-2561.2023.02.003
Outline
收藏切换

Agave spp. is main resource of hard fiber. Zebra disease, caused by the pathogen Phytophthora nicotianae, is one of the most devastating sisal oomycete diseases. RXLR effector proteins are important cytoplasmic effectors of oomycete and more than 300 RXLR effector genes were identified in the genomes of P. nicotianae. RXLR effectors have the ability to manipulate or suppress host immunity by directly binding host proteins. A typical RXLR effector (named PnRXLR5863) that induced programmed cell death (PCD) was identified from P. nicotianae, but the targets in Agave were still unknown. This study aims to obtain the host plant proteins that are interacting with PnRXLR5863 in A. hybrid No.11648. A normalized cDNA library was constructed by combining Switching Mechanism at 5′ end of RNA Transcript (SMART) and Duplex-Specific Nuclease (DSN) technology. The capacity of cDNA library was 5.32×107 CFU/ml, the independent clones was 1.064×108 CFU, and the lengths of inserts ranged from 0.5 kb to 3.0 kb, with the recombination rate of 100%. A total of 23 proteins interacting with PnRXLR5863 were obtained by sequencing and homology analysis. Some of target proteins involve several physiological events, such as ubiquitination, protein synthesis, protein translation and protein translation, suggesting that PnRXLR5863 effector may act on protein metabolism of Agave spp. This research would lay a foundation for further study of the pathogenic mechanism of PnRXLR5863.

RXLR effector protein  /  Agave hybrid No.11648  /  normalized library  /  protein interaction  /  zebra disease
Ziping YANG, Qian YANG, Xun WANG, Zhi KE, Zhiwei LU, Yanmei ZHANG, Huibang SHEN, Wenzhao ZHOU. Construction of Yeast Two-hybrid Normalized Library and Screening of Interaction Proteins of PnRXLR5863 in Agave hybrid No.11648[J]. Chinese Journal of Tropical Crops, 2023 , 44 (2) : 246 -253 . DOI: 10.3969/j.issn.1000-2561.2023.02.003
Appendix
Less
Year 2023 volume 44 Issue 2
PDF
39
12
Cite this Article
BibTeX
Article Info
doi: 10.3969/j.issn.1000-2561.2023.02.003
  • Receive Date：2022-05-10
  • Online Date：2026-03-05
  • Published：2023-02-25
Article Data
Affiliations
History
  • Received：2022-05-10
  • Revised：2022-06-24
Funding
Affiliations
    South Subtropical Crops Institute, Chinese Academy of Tropical Agricultural Sciences/Zhanjiang Key Laboratory of Tropical Crop Genetic Improvement, Zhanjiang, Guangdong 524091, China
References
Share
https://castjournals.cast.org.cn/joweb/rdzwxb/EN/10.3969/j.issn.1000-2561.2023.02.003
Share to
QR

Scan QR to access full text

Cite this article
BibTeX
Citations
表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
  • BibTeX
  • EndNote
  • RefWorks
  • TxT
Links
Articles
Latest Articles
Most Read
Collections
Updates
Events
News
Multimedia
About
About Us
Contact
No. 86 Xueyuan South Road, Haidian District, Beijing
100081
010-62199257
qkjq@cast.org.cn

Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House