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Peroxidase (POD) is a ubiquitous and highly active enzyme in plants, which plays an important role in plant growth and development and stress response. There are few reports on the analysis of POD family genes and the response to salt stress. In this study, 94 POD family members were identified in luffa genome by bioinformatics methods. They had similar gene structure, most of them contained 3-4 exons. The number of coding amino acids were ranged from 89 to 688, with an average of 309. The pI of the protein was between 4.58 and 10.34. 57 of them were basic amino acids. 82 members were hydrophilic proteins. POD family members were unevenly distributed on 13 chromosomes of luffa, of which chromosome 7 was the most (20) and chromosome 12 was the least (1). Phylogenetic analysis divided the POD family members into 8 subfamilies. The analysis of the collinearity of POD family genes with Arabidopsis showed that there were 12 pairs of collinearity genes between the two species, and the POD family genes in luffa had expanded. Protein conserved domain analysis showed that POD family members all contained plant peroxidases conserved domains and had similar conserved bases. The gene promoter contained a large number of plant hormone response elements such as abscisic acid, gibberellin, auxin, ethylene, salicylic acid, methyl jasmonate, and stress response cis elements such as low temperature, hypoxia, light, drought defense and stress response, indicating that it may be induced by abiotic stress. The analysis of cis acting elements in the promoter showed that the promoter contained a large number of phytohormone (abscisic acid, gibberellin, auxin, ethylene, salicylic acid, methyl jasmonate) response elements and stress (low temperature, low oxygen, light, drought, defense and stress response) response cis elements. When luffa seedlings were stressed by 200 and 500 mmol/L NaCl, the POD activity increased, and the gene expression of 56 POD family members increased significantly. Through expression trend analysis, two significant enrichment trends were obtained, including 35 genes that were significantly up-regulated under salt stress, indicating that the genes may participate in the response to salt stress. This study is the first time to identify the members of the POD family of luffa at the genomic level, and analyzed the physical properties and expression under salt stress. The results of this study would lay a theoretical foundation for exploring the biological functions of POD family members in luffa.
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| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
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