In order to provide theoretical and practical basis for the effective molybdenum deficiency control measures and rational application of molybdenum fertilizer, field investigation, morphological diagnosis, leaf nutrients content determination and spraying test of ammonium molybdate were carried out in navel orange (Citrus sinensis Osbeck). Further, navel orange leaves with different degrees of chlorisis were collected to measure photosynthetic pigment concentration, photosynthesis rate, chlorophyll fluorescence parameters and photosynthetic product concentration. For normal, slight chlorisis and severe chlorisis leaves, the concentration of nitrogen, potassium, magnesium and born were all in optimum range, zinc and calcium content were no significant difference. The molybdenum content of slight chlorisis and severe chlorisis leaves were lower than that of the threshold value of molybdenum deficiency (0.10 mg/kg), and decreased significantly than the normal leaves. Spraying 0.05% ammonium molybdate significantly reduced the symptoms of chlorisis, indicating that the yellowing leaves of navel oranges in the test orchard was caused by molybdenum deficiency. Molybdenum deficiency symptoms of navel orange were with nearly round and bright yellow waterlogged spots or patches on the new leaves, and the leaves were distorted with the edge leaf rolled up or inward. Photosynthetic pigment concentration, activity of RUBP carboxylase, potential activity (Fv/Fo), maximum photochemical efficiency (Fv/Fm), electron transfer efficiency (ETo/RC) and quantum yield (ETo/CSm) of PSⅡ reaction center decreased significantly with molybdenum deficiency, while the heat dissipation coefficient (DIo/RC) increased significantly. Molybdenum deficiency significantly decreased the photosynthetic rate (Pn), soluble sugar and sucrose contents, indicating molybdenum deficiency will decrease the photosynthesis of navel orange.
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |