Quickly obtain embryogenic callus is the key step to successfully establish the regeneration system in the somatic embryogenesis regeneration system of rubber tree. Rubber tree anthers were used as explants in the study. The effects of different concentrations of picloram, KT, 6-BA, and IAA on the induction of rubber tree anther embryogenic callus were studied through an orthogonal experimental design. The results showed that the optimal combination medium for rubber tree anther callus induction was MS basic medium, 0.4 mg/L thiamine, asparagine 300 mg/L, hydrolyzed casein 100 mg/L, proline 100 mg/L, arginine 100 mg/L, glutathione amino amide 100 mg/L, L-cysteine-hydrochloride 50 mg/L, sucrose 70 g/L, coconut water 50 mL/L, vegetable gel 2.2 g/L, picloram 20 mg/L, KT 2 mg/L and KT 2 mg/L and 6-BA 1 mg/L, and the callus induction rate reached 73.3%-100%. In all treatments, the callus was induced for about 25-28 days, and the callus reached the stage of rapid proliferation. The main effect of the induction of embryogenic callus was the concentration of picloram. It could promote the induction of embryogenic callus. Although low concentrations of picloram could also induce callus production, the callus were basically non-embryogenic potential-ie non-embryogenic callus. At the same time, 11 somatic embryos were used for plant regeneration. Among them, two plants emerged, seven only had roots without sprouting, and two had neither buds nor roots. The plant regeneration rate was 18%. By observing the leaf morphology and plant height of the plants, there was no significant difference with the rubber tree tissue culture seedlings and seedlings in production. In the future, on the basis of the research, the formulation of the callus induction medium would be further optimized, so that it could induce embryogenic callus from anthers more quickly, shorten the induction time of embryogenic callus, and reduce variation. Probability, it could provide callus with better conditions for RITA bioreactors as starting material.