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Cryopreservation of Anthurium andraeanum by Droplet Vitrification
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Jie GAO1, Zhiying LI2, Xuanbing ZHANG1, Longhai XIE2, Ying CHEN3, Zhenfen ZHU3, Yunliu FU4, Li XU5, *
Chinese Journal of Tropical Crops | 2023, 44(9) : 1909 - 1916
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Chinese Journal of Tropical Crops | 2023, 44(9): 1909-1916
• Post-harvest Treatment & Quality Safety •
Cryopreservation of Anthurium andraeanum by Droplet Vitrification
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Jie GAO1, Zhiying LI2, Xuanbing ZHANG1, Longhai XIE2, Ying CHEN3, Zhenfen ZHU3, Yunliu FU4, Li XU5, *
Affiliations
  • 1. Hainan University, Haikou, Hainan 570228, China
  • 2. Institute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
  • 3. Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Beijing 100000, China
  • 4. Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, Danzhou, Hainan 571737, China
  • 5. National Gene Bank of Tropical Crops, Danzhou, Hainan 571737, China
Published: 2023-09-25 doi: 10.3969/j.issn.1000-2561.2023.09.019
Outline
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Using the in vitro axillary bud of Anthurium (Anthurium andraeanum Lind.) as the materials, this article reports the influence of the droplet vitrification on Anthurium after cryopreservation, and plants regenerated tissues genetic stability were assessment. The results showed the highest survival rate of Anthurium (63.70%) was achieved after preculture for 4 days on MS solid medium containing 0.4 mmol/L sucrose and 2 mmol/L glycerol, dehydration with 80%PVS2 at 0 ℃ for 50 min, cooling in droplets of PVS2 placed on aluminum foil strips, after immersing in liquid nitrogen for 2 s, directly transferred it to a cryotube filled with liquid nitrogen, and kept it in liquid nitrogen for at least 30 min after rewarming them with MS liquid medium containing 1.2 mmol/L sucrose at room temperature for 20 minutes, transferred to recovery medium. Through ISSR and SSR molecular marker detection, the genetic stability of regenerated plants did not changed. The results would provide an effective way for long-term preservation of Anthurium andraeanum germplasm resources.

Anthurium andraeanum Lind  /  droplet vitrification  /  cryopreservation  /  axillary buds  /  genetic stability
Jie GAO, Zhiying LI, Xuanbing ZHANG, Longhai XIE, Ying CHEN, Zhenfen ZHU, Yunliu FU, Li XU. Cryopreservation of Anthurium andraeanum by Droplet Vitrification[J]. Chinese Journal of Tropical Crops, 2023 , 44 (9) : 1909 -1916 . DOI: 10.3969/j.issn.1000-2561.2023.09.019
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Year 2023 volume 44 Issue 9
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Article Info
doi: 10.3969/j.issn.1000-2561.2023.09.019
  • Receive Date：2022-04-09
  • Online Date：2026-03-05
  • Published：2023-09-25
Article Data
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History
  • Received：2022-04-09
  • Revised：2022-06-21
Funding
Affiliations
    1. Hainan University, Haikou, Hainan 570228, China
    2. Institute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
    3. Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Beijing 100000, China
    4. Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, Danzhou, Hainan 571737, China
    5. National Gene Bank of Tropical Crops, Danzhou, Hainan 571737, China
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https://castjournals.cast.org.cn/joweb/rdzwxb/EN/10.3969/j.issn.1000-2561.2023.09.019
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表12种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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