PDF
Full
Outline
Mangifera indica Linn. is a tropical and subtropical evergreen tree fruit, sweet fruit, rich nutrition, meat smooth and juicy, which is rich "tropical fruit king" reputation. In order to speed up the molecular identification of mango cultivars (lines), five mango cultivars (Red Ivory Mango, Jinhuang Mango, Red Mango No. 6, Red Jade Mango, Nan Doc Mai No. 4 Mango) with different phenotypic characteristics were selected from 19 test materials to screen SSR marker primers. The primers with better polymorphism were obtained by PAGE electrophoresis. Then, the primers were labeled with 5'-end modification with HEX and FAM fluorescent labels. The size of the amplified fragments were detected by capillary electrophoresis, and the polymorphic sites of each pair of primers were coded by numbers and letters. Finally, DNA fingerprint and DNA molecular ID card of mango were constructed by capillary electrophoresis. The results show that nine pairs of primers with good polymorphism were screened from the developed SSR primers from 115 pair primers through PCR amplification and PAGE electrophoresis. The primers were named M9, M15, M35, M40, M53, M59, M101, M102, and M103. After capillary electrophoresis detection and data analysis, bar code DNA molecular identification and quick response code DNA molecular identification of the mango cultivars (lines) were obtained combined with "number+letter" processing. For example, the fingerprint code of Jinhuang Mango is 9EE9F8933, the fingerprint code of Qianshan Mango No. 2 is H6F7564IC, and the fingerprint code of Qianshan Mango No. 4 is 3DGE47279. Finally, with the fingerprint code and basic information of 19 materials as the carrier, barcode DNA molecular ID and two-dimensional code DNA molecular ID were generated by online barcode generation program and two-dimensional code generation program. This study laid a foundation for standardizing the construction of molecular identification database of DNA of mango, and could provide data reference for mango genetics and breeding and excellent gene excavating.
PDF
49
12
| 科 Family | 属数 Number of genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) | 属 Genus | 种数 Number of species | 占总种数比例 Percentage of total species (%) |
|---|---|---|---|---|---|---|
| 鹅膏菌科Amanitaceae | 2 | 11 | 5.26 | 鹅膏菌属 Amanita | 10 | 4.78 |
| 小菇科 Mycenaceae | 2 | 12 | 5.74 | 丝盖伞属 Inocybe | 5 | 2.39 |
| 多孔菌科 Polyporaceae | 8 | 14 | 6.70 | 蜡蘑属 Laccaria | 5 | 2.39 |
| 红菇科 Russulaceae | 3 | 23 | 11.00 | 小皮伞属 Marasmius | 6 | 2.87 |
| 小菇属 Mycena | 11 | 5.26 | ||||
| 光柄菇属 Pluteus | 5 | 2.39 | ||||
| 红菇属 Russula | 17 | 8.13 | ||||
| 栓菌属 Trametes | 5 | 2.39 |
关闭全屏
No. 86 Xueyuan South Road, Haidian District, Beijing
010-62199257
qkjq@cast.org.cn
Copyright © 2025 China Association for Science and Technology. All rights reserved. For all open access content, the relevant licensing terms apply.
Sponsored by the Office of the Leading Group for Cybersecurity and Informatization of CAST, and supported by Science and Technology Review Publishing House
